D-松醇对二型糖尿病大鼠PI3K及下游糖原合成基因的调控

Effect of D-pinitol on regulating the downstream glycogen synthesis gene and PI3K/Akt of T2DM rats

主要探究D-松醇对2型糖尿病大鼠PI3K及下游糖原合成基因的调控作用,选用雄性SD大鼠进行实验。高脂高糖饲料喂养配合注射链脲佐菌素(STZ)诱导建立2型糖尿病模型。将实验动物分为正常对照组(N)、模型对照组(C)、D-松醇低剂量组(L,30mg/(kg BW·d))、D-松醇高剂量组(H,60mg/(kg BW·d))和正常高剂量组(NH,60mg/(kg BW·d))。N组和NH组饲喂正常饲料,其余3组饲喂高脂高糖饲料,灌胃治疗期间定期进行体重和空腹血糖(FBG)测定,5周后大鼠处死并对其糖化血清蛋白(GSP)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)以及肝糖原等生化指标进行测定,并从肝脏中提取m RNA,利用PCR技术对大鼠肝脏中的PI3K、Akt、GSK-3β和GS的表达进行检测。结果表明:经灌胃治疗5周后,H组与C组相比FBG、GSP、AST和ALT水平均极显著性降低(p<0.01),肝糖原含量极显著性增加(p<0.01),L组和H组PI3K、Akt和GS的表达上调,GSK-3β表达下调,说明D-松醇可以通过调控大鼠肝脏PI3K及下游糖原合成基因来调节患糖尿病大鼠的血糖,起到降血糖的作用。

Effect of D-pinitol on regulating the downstream glycogen synthesis gene and PI3K/Akt of T2 DM rats was studied in this investigation. A model of type 2 diabetes mellitus was established by feeding a high-fat diet（HFD）and injecting streptozocin（STZ）to the Sprague Dawley（SD）rats. Experimental animals were divided into 5 groups,including the normal group（N）,control group（C）,low-dosage group of D-pinitol（L,30mg/（kg BW·d））,high-dosage group of D-pinitol（H,60mg/（kg BW·d））and normal high-dosage control group（NH,60 mg/（kg BW·d））. N and NH groups were fed with a normal chow diet and the others were fed with a HFD. During the experimental period,the rats were administrated with the tested sample each day and body weight and fasting blood glucose（FBG）were tested. The contents of GSP,AST,ALT and hepatic glycogen were determined after fiveweek-treatment. The m RNA was extracted from liver to investigate the expressions of PI3 K,Akt,GSK-3β and GS through the reverse transcription polymerase chain reaction（RT-PCR）. The results showed that the levels of FBG,GSP,AST and ALT in H group were very significantly lowered compared with C group（p 0.01）,while the contents of hepatic glycogen were increased with very significance（p 0.01）after five-week-treatment. The results of RT-PCR showed that D-pinitol could improve the expressions of PI3 K,Akt and GS,and inhibit the expression of GSK-3β in gene level,which suggested that D-pinitol has a hypoglycemic effect through regulating the downstream glycogen synthesis gene and PI3K/Akt of T2 DM rats.