摘要
以甲醇、95%乙醇、乙酸乙酯、丙酮、三氯甲烷等不同极性的溶剂对半枝莲进行初步提取,以亚硝酸钠-硝酸铝-氢氧化钠法、福林-酚法、二苯代苦味酰基自由基法(DPPH法)、铁离子还原法(FRAP法)分别测定粗提物的总黄酮含量、总酚含量、自由基清除能力、总还原力大小。结果显示,以甲醇为溶剂的超声提取物总酚含量最高,为104.39mg/g提取物。采用多种溶剂对半枝莲甲醇超声粗提物进行顺序萃取,其中乙醚萃取物的抗氧化活性较高,总黄酮、总酚含量分别为11.210、105.392mg/g萃取物,DPPH自由基清除能力(EC50)为0.15mg(以甲醇粗提物质量计),总抗氧化值0.468、2.072mmol。结合HPLC分析可知,采用不同极性溶剂对甲醇超声粗提物进行萃取,可对其抗氧化活性物质进行初步分离纯化。
The powders of whole plant of Scutellaria barbata were extractedby methanol,95% ethanol,acetone and chloroform method. The contents of total flavonoid and total phenol were measured by aluminum chloride and FolinCiocalteu colorimetric assays,respectively. The antioxidant activitiesin vitroof extractswere investigated by DPPH radical-scavenging assay and ferric reducing/antioxidant power（FRAP）assay. The crude extract by methanol had the highest content of total phenol — 34.618mg/g（of extraction）. The sequential extraction by ethyl ether had the highest value of antioxidant activity. Its total flavonoid,totalphenol,DPPH radical-scavenging activity（EC50）and ferric reducing power were as high as11.210mg/g（of sequential extraction）,105.392mg/g（of sequential extraction）,0.15mg（of extraction）and 0.468,2.072 mmol. HPLC analysis showed that antioxidant active components of the crude extraction could be purified and analyzed by sequential extraction with different polar solvents.
出处
《中国食品添加剂》
CAS
北大核心
2015年第9期72-77,共6页
China Food Additives
基金
广东省普通高校人文社会科学省市共建重点研究基地招标项目(12KYKT10)
广东省教育厅"高等教育教学改革"项目(GDJG20142453)
梅州市科技局
嘉应学院联合资助项目(2012KJA11)
关键词
半枝莲
粗提物
顺序萃取
抗氧化活性
HPLC
scutellaria barbata
crude extract
sequential extraction
antioxidant activity
HPLC
