外周血CK-20基因表达与大肠癌微转移关系的研究

Relationship between CK-20 expression in peripheral blood of colorectal cancer patients and tumor micrometastasis

目的 :探讨靶基因CK - 2 0在直肠癌患者外周血中的表达及与肿瘤微转移的关系。方法 :应用套式RT -PCR方法检测 1 0例健康成人 ,1 7例非恶性消化系统疾病患者 ,32例大肠癌患者外周血中的肿瘤细胞。结果 :①正常对照组及非大肠癌对照组均未检出CK - 2 0mRNA阳性细胞 ;②大肠癌组中 ,2例A期患者CK - 2 0mRNA均呈阴性 ,1 5例B期患者中阳性 4例 ;1 0例C期患者中阳性 6例 ;5例D期患者中阳性 4例 ;③在 32例大肠癌患者中CK- 2 0mRNA阳性总检出率为 43 8% ;A、B期阳性率为 2 3 5 % ;C、D期阳性率为 66 7%。结论 :应用RT -PCR法检测外周血瘤细胞的CK - 2 0靶基因对大肠癌具有特异、敏感、无损伤等特点 。

AIM: To explore the expression of cytokeratin 20 (CK-20)target gene in peripheral blood of patients with colorectal cancer. METHODS: Total cellular RNA was extracted from whole blood of cancer patients and control groups, including 10 healthy adults, 17 benign gastrointestinal disease patients, 32 cases of colorectal cancer. The CK-20 gene expression was measured by reverse transcription PCR. RESULTS: 1. CK-20 mRNA was not detected in benign gastrointestinal disease patients and healthy adults. 2. In the colorectal carcinoma group, CK-20 mRNA expression was negative in 2 case of patients at Dukes A. The CK-20 mRNA positive expression can be detected in 4 patients among 15 cases at Dukes B, 6 out of 10 cases at Dukes C, 4 out of 5 cases at Dukes D. 3. The total positive percentage of CK-20 mRNA is 43 8% in 32 colorectal cancer patients; The positive percentage of CK-20 mRNA in patients at Dukes A and B is 23 5% and 6 7% in Dukes C, D patients CONCLUSION: It is a specific, sensitive and no damage method to detect the CK-20 target gene in patients with colorectal cancer. It is helpful for early diagnosis of tumor micrometastasis and for direction of chemotherapy.