端粒酶RNA反义核酸降低鼻咽癌细胞p53蛋白表达水平

Decreased expression of p53 protein in nasopharyngeal carcinoma cells by telomerase RNA antisense oligodeoxynucleotides

目的 :研究端粒酶活性抑制后鼻咽癌细胞p53蛋白表达水平的变化。方法 :脂质体介导端粒酶反义寡核苷酸转染鼻咽癌CNE1和CNE2Z细胞株 ,MTT法检测细胞增殖能力 ,流式细胞术检测细胞凋亡 ,免疫组化法检测端粒酶逆转录酶 (hTRT)和p53蛋白表达水平。结果 :端粒酶RNA反义寡核苷酸可抑制CNE1和CNE2Z细胞的增殖并呈剂量依赖性 ,流式细胞仪检测出现凋亡峰 ,反义寡核苷酸组hTRT和p53蛋白表达水平显著低于其它处理组及空白对照组 (P <0 .0 1 )。结论

AIM: To study the expression of p53 protein in nasopharyngeal carcinoma cells(NPC) after the telomerase activity being inhibited. METHODS: After transfecting telomerase RNA antisense oligodeoxynucleotides (asODN) into NPC CNE1 and CNE2Z cells by lipofectin, the proliferation of cells was identified by MTT method, apoptosis was examined by flow cytometric cell cycle analysis, and the expression levels of human teolmerase reverse transcriptase(hTRT) and p53 protein were detected by immunohistochemistry. RESULTS: asODN inhibited the growth of CNE1 and CNE2Z cells in a dose-dependent manner, meanwhile, the apoptotic peaks were detected with flow cytometer.The expression levels of hTRT and p53 protein in asODN group were significantly lower than those in other treatment groups and control group( P <0.01).CONCLUSION: The telomerase RNA asODN which inhibits the telomerase activity could decrease the expression of p53 protein in NPC.