偶氮染料脱色菌Lysinibacillus sp.FS1的脱色性能

Biodecolorization of azo dyes by Lysinibacillus sp. FS1

从佛山某工业园印染废水处理厂曝气池中,经梯度驯化筛选出一株对多种染料具有较强脱色能力的菌株FS1,通过16S r DNA基因序列分析初步鉴定为Lysinibacillus sp.,研究了该菌株在不同营养条件（氮源、碳源、碳源浓度）,不同培养条件（p H、温度、供氧条件）,不同染料（甲基橙、亚甲基蓝、中性红、酸性红B）和染料浓度下的脱色性能。结果表明,该菌株的最佳脱色条件：温度30~40℃,p H 7~9,氯化铵1 g/L,葡萄糖2 g/L的厌氧条件下培养脱色效果最好,10 h时对酸性红B脱色率可达98.73%左右,且脱色过程符合一级反应动力学方程：-ln（At/A0）=0.0588t-0.0448。该菌株可降解多种染料,脱色率均随污染强度的升高先增大后减小,对高浓度染料和混合染料也表现出很好的脱色效果,是一株高效广谱的染料降解菌,具有处理印染废水的开发应用价值。

A strain was isolated through gradient acclimation from the aeration pool of a dye wastewater treatment plant in Foshan industrial park,which could decolorize various dyes efficiently. The strain was identified and named as Lysinibacillus sp. FS1 based on 16 S r DNA gene sequence analysis. Affecting factors of decolorization were investigated,including nutritional conditions（ nitrogen sources,carbon sources,carbon concentration）,culture conditions（ p H,temperature,oxygen conditions）,types and concentrations of dyes（ methyl orange,methylene blue,neutral red and acid red B）. The result demonstrated that the optimal conditions of decolorization were30—40℃,p H 7—9 with 1 g / L chloride as nitrogen source,2 g / L glucose as carbon source. It could anaerobically decolorize almost 98. 73% acid red B in 10 h,and the decolorization process of FS1 fits the first-class kinetics model：-ln（ At/ A0） = 0. 0588t-0. 0448. The strain can be a high-efficiency biological degradant,even for high concentrated dyes and mixed dyes,and the variation of its decolorization rate has a unimodal curve with the increase of pollution intensity. It comes to a conclusion that the strain FS1 can be practically used in wastewater treatment.