厌氧氨氧化反应过程的三维荧光光谱解析

Analysis of EEM fluorescence spectra of effluents from ANAMMOX reactor

采用三维荧光光谱表征了厌氧氨氧化UASB反应器启动过程中和有机冲击负荷条件下的出水样品,应用平行因子分析方法解析样品中各主成分的三维荧光光谱图。研究结果表明,反应器启动阶段,出水中含有类蛋白质、类富里酸和类腐殖酸物质,主要源于接种污泥的菌体自溶和残留有机物厌氧发酵。随着反应器的运行,出水中的类蛋白质,类富里酸物质和类腐殖酸荧光物质逐渐减少。在有机冲击负荷条件下,出水中的类蛋白质组分迅速增加并成为反应器出水中的主要荧光物质。该研究为厌氧氨氧化的启动及运行控制的监控表征提供了新的方法。

The effluents were characterized by excitation-emission matrix（ EEM） fluorescence spectra from different operation conditions such as the start-up phase,and the organic loading shock phase. Parallel factor analysis was used to decompose the fluorescent components of the samples and their EEM fluorescence spectra were acquired. Results showed that there were protein-like,fulvic-like and humic-like substances in the effluents of the start-up phase,which was derived from the cell autolysis and anaerobic fermentation of inoculated sludge. With the operation of ANAMMOX reactor,the protein-like,the fulvic-like and humic-like substance decreased gradually. The protein-like substance increased rapidly and became the main fluorophore of the effluents under the organic shock loading conditions. This work provides a new monitoring method for the start-up and operational control of the ANAMMOX reactor.