忍冬开花过程中花青苷组成及含量的变化

Changes in composition and content of anthocyanins during flowering process of Lonicera

【目的】探讨忍冬属(Lonicera Linn.)植物花朵中花青苷的种类及开花过程中含量的变化,为忍冬花色育种提供依据。【方法】以忍冬(L.japonica Thunb.)、‘火焰’忍冬(L.×heckrottii Rehd.‘Huoyan’)、‘格雷姆’忍冬(L.periclymenum L.‘Geleimu’)、贯月忍冬(L.sempervirens L.)和‘台尔曼’忍冬(L.×tellmanniana)5种藤本植物为材料,采用高效液相色谱-二极管阵列检测技术(HPLC-DAD)、高效液相色谱-电喷雾离子化-质谱联用技术(HPLC-ESI-MSn),对各材料开花过程中的花青苷进行定性及定量分析。【结果】在开花过程中,5种忍冬属植物花色变化明显,但在忍冬花朵中未检测到花青苷存在,在‘火焰’忍冬、贯月忍冬、‘格雷姆’忍冬和‘台尔曼’忍冬中共检测到4种花青苷,分别为矢车菊素3,5-二葡萄糖苷、矢车菊素3-葡萄糖苷、芍药花素3,5-二葡萄糖苷和芍药花素3-葡萄糖苷,其中矢车菊素3,5-二葡萄糖苷为主要花色素成分。5种忍冬属植物在不同开花阶段花青苷种类基本一致,但含量均有较为明显的变化。【结论】5种忍冬属植物的花青苷组成及含量变化与其花色转变相一致,可为忍冬花色育种提供参考。

【Objective】This study investigated the changes in anthocyanins composition and content in Lonicera during flowering to provide theoretical basis for development and breeding of flower colors.【Method】A high performance liquid chromatography（HPLC）with a photodiode array detector（HPLCDAD）and a HPLC-electrospray ionization-multiple mass spectrometry（HPLC-ESI-MSn）were employed for qualitative and quantitative analysis of anthocyanins from L.japonica Thunb.,L.×heckrottii Rehd.‘Huoyan＇,L.periclymenum L.‘Geleimu＇,L.sempervirens L.and L.×tellmanniana.【Result】During flowering process,the colors of all varieties changed significantly.But no anthocyanins were detected in flowers of L.japonicaduring flowering process.In L.×heckrottii Rehd.‘Huoyan＇,L.sempervirens L.,L.periclymenum L.‘Geleimu＇and L.×tellmanniana,a total of 4anthocyanins including Cy 3,5-di-O-glucoside,Cy 3-O-glucoside,Pn 3,5-di-O-glucoside,and Pn 3-O-hexoside were detected.Cy 3,5-di-O-glucoside was the main pigment.The types of anthocyanins from different varieties were almost same but their con-tents were significantly different.【Conclusion】The transformation of flower colors was consistent with composition and content of anthocyanins in Loniceraflower.This study could provide reference for breeding and selection of special flower colors.