中心组合设计-响应曲面法优化淫羊藿苷超声波提取工艺

Optimization of icariin extraction from Epimedium brevicornu maxim by supersonic wave using central composite design and response surface methodology

【目的】采用中心组合设计-响应面法优化淫羊藿苷的超声波提取工艺。【方法】在单因素试验的基础上采用中心组合设计方法,研究了乙醇用量、超声提取时间、液(mL)固(g)比及其交互作用对淫羊藿苷提取率的影响。【结果】通过岭脊分析确定的淫羊藿苷最优提取条件为:乙醇体积分数68%、超声提取时间23min、液(mL)固(g)比32∶1,该条件下淫羊藿苷的提取率可达到0.661%,与理论值0.663%十分接近。【结论】确定了淫羊藿苷的最佳提取工艺,该工艺具有耗时短、提取率高、能量消耗少且工艺稳定可靠等优点。

【Objective】The ultrasound-assisted extraction of icariin fromEpimedium brevicornu Maxim was optimized using response surface methodology and central composite design（CCD）.【Method】Based on single factor tests,the effects of ethanol concentration,ultrasonic extraction time,liquid to solid ratio and their interactions on extraction rate were studied using CCD method.【Result】The optimal ultrasonic extraction conditions based on ridge analysis were：ethanol concentration 68%,ultrasonic extraction time 23 min,liquid to solid ratio 32mL/g.Under the optimum conditions,the extraction yield of total icariin was 0.661%,which was close to the theoretical value of 0.663%.【Conclusion】The optimal extraction conditions of epimedium glycoside extraction were determined and the advantages included short time,high extraction efficiency,less energy consumption,stable,and reliable.