天然虾青素的抗疲劳及抗氧化作用研究

Study on the Anti-Fatigue and Anti-Oxidant Effects of Natural Astaxanthin

为了验证天然虾青素的抗疲劳和抗氧化活性,采用乙醇萃取法提取虾壳中的天然虾青素.以KM小鼠为实验动物,连续给药30d,测量体质量变化;通过小鼠负重游泳实验,测量小鼠力竭游泳时间和肝脏组织的抗氧化酶系统活性(T-SOD,GSH-Px,T-AOC,MDA,NO);测量小鼠20min定时游泳后,血液中的BLA,BUN,LG,MG等4个指标的变化情况,和血液中的血糖浓度及抗氧化酶系统活性;根据线性方程,分析天然虾青素抗疲劳和抗氧化活性的关系.结果表明,天然虾青素可增加小鼠力竭游泳时间,降低游泳运动后小鼠机体内BLA和BUN浓度,提高LG,MG和血糖浓度;提高肝脏和血液中的T-SOD,GSH-Px和T-AOC等活性指标,降低MDA和NO浓度;且相关分析发现,小鼠体内随着T-AOC活性升高,BLA,BUN浓度减少,LG,MG浓度升高.天然虾青素具有显著的抗氧化和抗疲劳作用,且抗氧化能力和抗疲劳能力呈正相关,两者相辅相成.

Objective：To verify the anti-fatigue and anti-oxidant activity of natural astaxanthin.Methods：Extracting natural astaxanthin from shrimp with ethanol.Choosing KM mice as the experimental animals and dosing continuously lasting up to 30 days and recording their weight changes.Measuring exhaustion swimming time and activity of the anti-oxidant enzyme system（T-SOD,GSH-Px,T-AOC,MDA and NO）of the liver tissue of the mice through loaned-swimming test.Determining the changes of BLA,BUN,LG and MG in the blood and measuring blood sugar levels and the activity of the antioxidant enzyme system after 20 min swimming.According to the linear equation,analyzing the relationship between antifatigue and anti-oxidant activities of natural astaxanthin.Results：Natural astaxanthin could increase the swimming time of mice,reduce BLA and BUN levels after swimming,improve LG and MG,and blood sugar levels,and raise the contents of T-SOD,GSH-Px and T-AOC and other activity indicators in the liver and the blood,and reduce the contents of MDA and NO.Correlation analysis showed that the contents of BLA and BUN were reduced and the contents of LG and MG increased with increasing T-AOC activity.Conclusion：Natural astaxanthin has significant anti-oxidant and anti-fatigue effect,and anti-oxidant capacity is positively associated with anti-fatigue capacity.