正交实验法优化艾纳香提取废渣中总黄酮提取工艺

Optimization of extraction process of total flavonoids from Blumea balsamifera residue by orthogonal test

采用三氯化铝显色,芦丁作对照品,紫外分光光度法测定总黄酮含量,对艾纳香叶中总黄酮提取因素进行研究,采用单因素筛选结合L9(34)正交试验法优选艾纳香总黄酮提取参数。结果表明,提取艾纳香黄酮因素影响大小为:料液比(C)>提取温度(B)>提取时间(D)>提取溶剂乙醇体积分数(A)>提取次数,最佳提取工艺参数为:60%乙醇、温度90℃、料液比1∶20、提取3 h,总黄酮百分含量为2.29%。经提取验证,该提取工艺对艾纳香总黄酮资源化开发与利用有一定的技术参考价值。

Taking the content of total flavonoids as investigated index,solid-liquid ratio,extraction temperature,extraction duration,extraction times and ethanol concentration as factors,on the basis of single factors,the extraction process of total flavonoids from Blumea balsamifera was optimized through L9（34）orthogonal design. The results showed that factors influencing total flavonoids from B. balsamifera were as followings ：solid-liquid ratio（C）〉 temperature（B）〉 extraction duration（D）〉 alcohol concentration（A）〉 extraction times. The optimal extraction conditions were 60% alcohol, 90℃,1∶20 solid-liquid ratio,for 3 h. Under these conditions,the flavonoids content reached 2.29%. The extraction process provided a reference for development and utilization of total flavonoids in B. balsamifera.