地衣芽胞杆菌对实验性结肠炎相关肝脏病变的疗效及机制影响

Efficacy of Bacillus licheniformis in mice with colitis related liver disease

目的:探讨地衣芽孢杆菌对实验性结肠炎相关肝脏病变的治疗作用与机制.方法:将45只BALB/c小鼠随机分为正常组、葡聚糖硫酸钠(dextran sulfate sodium,D S S)组和治疗组,实验过程中对各组小鼠进行疾病活动指数评分(disease activity index,DAI),在造模及给药后第11天处死小鼠,检测血清丙氨酸氨基转移酶(aspartate transaminase,ALT)、天门冬氨酸氨基转移酶(alanine aminotransferase,AST)、D-乳酸、二胺氧化酶(diamine oxidase,DAO)、内毒素(endotoxin),取结肠及肝脏进行HE染色,运用酶联免疫吸附测定(ELISA)肝组织匀浆中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)含量水平.结果:通过地衣芽孢杆菌对DSS模型小鼠进行治疗发现,与正常组比较,DSS组DAI评分明显增高(P<0.05),治疗组与DSS组相比DAI评分降低(P<0.05);DSS组结肠长度与治疗组相比缩短程度更为显著(P<0.05);与正常组比较,DSS组及治疗组血清ALT、AST、D-乳酸、D A O、内毒素均升高(P<0.05),肝组织匀浆中TNF-α、IL-6含量显著升高(P<0.05);而治疗组血清A LT、A S T、D-乳酸、DAO、内毒素较DSS组均降低(P<0.05),肝组织匀浆中TNF-α、IL-6含量较DSS组显著降低(P<0.05).结论:地衣芽孢杆菌对实验性结肠炎及相关肝胆病变有明显治疗作用,可能与改善肠黏膜通透性和恢复免疫功能有关.

AIM：To investigate the therapeutic effects of Bacillus licheniformis on experimental colitis associated hepatobiliary disease.METHODS：Forty-five male BALB/c mice were randomly divided into three groups：a normal group,a model group and a treatment group.Experimental colitis was induced in mice with dextran sulfate sodium.Disease activity index（DAI） was evaluated daily during the study period.Serum levels of aspartate transaminase（ALT）,alanine aminotransferase（AST）,D-lactate,diamine oxidase（DAO）,and endotoxin were measured.The levels of tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in liver tissue were measured by ELISA.RESULTS：Compared with the normal group,DAI score was significantly higher in the DSS group（P〈0.05）;however,DAI score was significantly lower in the treatment group compared with the DSS group（P〈0.05）.Colon shortening was more significant in the DSS group than in the treatment group（P〈0.05）.Compared with the normal group,serum levels of ALT,AST,D-lactate,DAO,and endotoxin as well as the levels of TNF-α and IL-6 in liver homogenates were significantly increased（P〈0.05） in the DSS group;these parameters were significantly lower in the treatment group than in the DSS group（P〈0.05）.CONCLUSION：Bacillus licheniformis can reduce the severity of colitis associated liver disease possibly by promoting the recovery of intestinal mucosal permeability and immune function.