Eotaxin-3在嗜酸性粒细胞胃肠炎中的表达及糖皮质激素对其表达的影响

Effect of glucocorticoid on expression of Eotaxin-3 in eosinophilic gastroenteritis

目的:探讨嗜酸性粒细胞趋化因子-3(eosinophil chemotactic factor-3,Eotaxin-3)在嗜酸性粒细胞胃肠炎(eosinophilic gastroenteritis,EG)患者中的表达及糖皮质激素对其表达的影响.方法:选取首次确诊的EG患者48例,随机平均分为未治疗组与强的松(Prednisone)治疗组,以同期的24例健康者为对照组,使用酶联免疫吸附测定法(enzyme-linked immunosorbent assay)分别测定研究对象其血清及组织中Eotaxin-3的表达.结果:EG各组治疗前同对照组相比,血清及组织中Eotaxin-3的表达水平明显增高(P<0.05);未治疗组服用安慰剂前后的血清及组织中Eotaxin-3的表达水平差异无统计学意义,比较治疗组的治疗前后,血清及组织中Eotaxin-3的表达水平均有明显下降(P<0.05);EG各组组织中及血清中Eotaxin-3的表达水平与组织中嗜酸性粒细胞(eosinophil)计数均呈正相关关系(r=0.96、r=0.94,均P<0.01).结论:Eotaxin-3可能参与了EG的病理生理过程,激素治疗EG可能通过抑制Eotaxin-3的表达水平实现的,这对EG的临床诊治有一定的指导意义.血清中Eotaxin-3作为非侵入性指标评价EG的治疗效果有一定的参考价值.

AIM：To detect the expression of eosinophil chemotactic factor-3（Eotaxin-3） in eosinophilic gastroenteritis（EG） and investigate the effect of glucocorticoid on Eotaxin-3 expression.METHODS：Forty-eight patients with EG and 24 healthy controls were included.Patients with EG were equally divided into two groups,a placebo group and a prednisone group.Eotaxin-3expression was measured by enzyme-linked immunosorbent assay in the serum and tissue samples of all subjects.RESULTS：The expression levels of Eotaxin-3in serum and tissue were significantly elevated in the EG group compared to normal controls（P〈0.05）.Eotaxin-3 in serum and tissue significantly decreased after treatment（P〈0.05）,although its change had no significance in the placebo group.A positive correlation was observed between eosinophil（Eos） count and Eotaxin-3 in serum and tissue in the EG group.CONCLUSION：Eotaxin-3 may be involved in the pathophysiological process of EG.Hormone therapy may inhibit the expression of Eotaxin-3.Serum Eotaxin-3 may be used as a noninvasive index for evaluating the treatment effect in EG.