高效液相色谱法检测板蓝根颗粒消炎成分分析

Determination of anti-inflammatory constituents in Radix Isatidis granules by high-performance liquid chromatography

目的：探讨建立检测板蓝根颗粒消炎成分的高效液相色谱法（HLPC法）。方法：利用HLPC检测板蓝根颗粒中消炎成分腺苷的含量,色谱条件：采用Waters Symmetry C18液相色谱柱（150×4.6mm,5μm）,流动相为甲醇∶醋酸∶水（10∶10∶80）进行梯度洗脱,流速为1.2mL/min,柱温为30℃,检测波长为260nm,进样量为15μL。结果：板蓝根颗粒中腺苷进样量与峰面积线性关系良好（r=0.9998）的范围为0.0250-0.1550μg,精密度试验中RSD为0.78%、重现性试验中RSD为0.69%;平均加样回收率为99.54%（RSD=0.82%）;腺苷含量为0.0700mg/g（RSD=0.78%）。结论：HLPC检测板蓝根颗粒消炎成分的方法精密度、重现性良好,操作简便、快捷,测定结果准确,可作为板蓝根颗粒制剂质量控制标准的检测方法。

Objective： To establish a high-performance liquid chromatography（ HLPC） method for the determination of anti-inflammatory constituents in Radix Isatidis granules. Methods： To determine the content of adenosine,one of anti-inflammatory constituents in Radix Isatidis granules,HLPC was performed on a Waters Symmetry C18column（ 150 × 4. 6mm,5m） using methanol-acetic acid-water（ 10∶ 10∶ 80） as a mobile phase for gradient elution at a flow rate of 1. 2 ml / min,a column temperature of 30° C,a detection wavelength of 260 nm,and a sample size of 15 μL. Results： The content of adenosine in Radix Isatidis granules showed a good linear relationship with the peak area within 0. 0250-0. 1550 μg（ r = 0. 9998）,with a relative standard deviation（ RSD） of 0. 78% in precision test and 0. 69% in repeatability test; the average recovery rate was 99. 54%（ RSD = 0. 82%）; the content of adenosine was 0. 0700 mg / g（ RSD = 0. 78%）. Conclusion： The HLPC method for determination of anti-inflammatory constituents in Radix Isatidis granules is highly precise and repeatable,accurate,and easy to operate and can be used for the quality control of Radix Isatidis granules.