摘要
目的观察孟根乌森乌日乐对咪喹莫特诱导的银屑病样皮损的治疗作用及其机制。方法 BALB/c雌性小鼠50只,随机分为正常对照组、模型组、低、中、高3个剂量组,每组各10只。模型组及低、中、高3个剂量组小鼠背部涂抹咪喹莫特乳膏21 mg,每天1次,连续7 d;正常对照组小鼠背部涂抹凡士林21mg,每天1次,连续7 d。正常对照组予以0.3%羧甲基纤维素钠2 m L/100 g,每天一次,灌胃给药;模型组予以0.3%羧甲基纤维素钠2 m L/100 g,每天一次,灌胃给药;低、中、高3个剂量组分别予以0.33,0.66,1.32 g·kg-1孟根乌森乌日乐,每天一次,灌胃给药。3组均连续给药21 d。用免疫组织化学法检测皮损组织的增殖细胞核抗原、转化生长因子-α、肿瘤坏死因子-α、血小板内皮细胞黏附分子、血管内皮生长因子、Toll样受体2。结果低、中、高3个剂量组的6种免疫组织因子均高于模型组,且高剂量的效果好于中剂量,中剂量的效果好于低剂量。结论孟根乌森乌日乐对咪喹莫特诱导小鼠银屑病样皮损有较好的治疗作用。
Objective To investigate the efficacy and mechanism of mongolian medicine Menggenwusenrile in the treatment of imiquimod - induced psoriasiform skin inflammation in mice. Methods Fifty cases of BALB/c female mice were randomly divided into normal control group, model group, Menggenwusenrile low, middle and high dose groups, 10 cases in each group. Normal control group was given 0. 3% sodium car- boxyl methyl cellulose（CMCNa） 2 mL/100 g, once a day intragastric administration for consecutive 21 days. Modal group was given O. 3% CMCNa 2 mL/100 g, once a day intragastric administration for consecu- tive 21 days. Menggenwusenrile low, middle and high dose groups were given 0.33, 0. 66, 1.32 g ~ kg-1 , once a day intragastric administration for consecutive 21 days. The expression of proliferating cell nuclear anti- gen （PCNA）, transforming growth factor-or （TGF- c~）, tumor necrosis factor - ~x （TNF - ~） , platelet endothelial cell adhesion molecule - 1/ CD31 （ CD31 ）, vascular endothelial growth factor （VEGF） and Toll - like receptors- 2 （TLR -2）were detected by immunohistoehemieal stai- ning. Results Six kinds of immunohistochemical analysis showed the each drug dose group was higher than the model group, high dose is bet- ter than middle dose group, the middle dose group is better than low dose. Conclusion Menggenwusenrile have a better therapeutic effect in imiquimod - induced psoriasiform skin inflam- mation in mice.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第20期2040-2042,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家"十二五"科技支撑计划基金资助项目(2012BAI27B0204)
