抗坏血酸对岩黄连愈伤组织褐化及抗氧化酶活性的影响

Effect of Ascorbic Acid on Browning and Activity of Antioxidant Enzyme of Corydalis saxicola Bunting Callus

以岩黄连愈伤组织为试材,采用固体培养方法,研究不同浓度的抗坏血酸对岩黄连愈伤组织褐化及抗氧化酶活性的影响。结果表明:培养第20天添加抗坏血酸的各处理岩黄连愈伤组织的褐化率均低于对照,其中抗坏血酸浓度为175mg/L的处理岩黄连愈伤组织的褐化率最低,其褐化率仅为18.5%;在整个培养阶段,添加抗坏血酸的各处理岩黄连愈伤组织的抗氧化酶活性均比对照的高,在培养第30天各处理岩黄连愈伤组织的POD和SOD活性均为最高值,其中抗坏血酸浓度为175mg/L的处理岩黄连愈伤组织的POD和SOD活性均为最高,分别达到35.24U·g-1·min-1、162.79U·g-1;在培养第20天添加抗坏血酸的各处理CAT活性均为最大值,分别比对照高了2.28、3.21、5.33、3.51U·g-1·min-1。

The effect of different ascorhic acid concentration on browning and activity of antioxidant enzyme of Corydahs saxicola Bunting callus were studied in this experiment of which Corydalis saxicola Bunting callus was used as the materials and was cultured on solid media. The results showed that the browning rate of Corydalis saxicola Bunting callus of treatments with different ascorbic acid concentration were lower than that of the control after 20 days culturing, and the browning rate of the treatment with 175 mg/L concentration of ascorbic acid was the lowest with 18. 5%. The activity of antioxidant enzyme of Corydalis saxicola Bunting callus of the treatments with different ascorbic acid concentration were higher than that of the control throughout the culturing phase, the activities of POD and SOD reached the highest value after 30 days culturing,and which of the treatment with 175 mg/L concentration of ascorbic acid were the highest with 35.24 U· g-1 · min-1 and 162. 79 U · g-1. Also the activity of CAT of the treatments with different ascorbic acid concentration reached the highest value after 30 days culturing,which were higher than that of the control with 2. 28 U · g-1 . min-1,3. 21 U · g-1 . min-· ,5. 33 U · g-1 . min-1 ,3. 51 U · g-1 . min-1 respectively.