应用羧基荧光素-寡核苷酸-羧基四甲基罗丹明荧光探针测量吸收剂量

Application of carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine fluorescent probe in dosimetry

用X-射线照射不同浓度羧基荧光素-寡核苷酸-羧基四甲基罗丹明(Carboxyfluorescein-Oligonucleotidecarboxytetramethylrhodamine,FAM-DNA-TAMRA)水溶液,测量溶液发出的荧光强度,研究吸收剂量与荧光强度之间的关系。结果表明,对于含不同浓度FAM-DNA-TAMRA荧光探针的水溶液,在0-20 Gy剂量范围内,荧光强度与吸收剂量之间的线性关系很好,并且荧光稳定性良好,但存在荧光本底比较高,影响测量吸收剂量灵敏度的问题。研究结果提示,FAM-DNA-TAMRA荧光探针可以用于测量水溶液的吸收剂量。

To explore the relationship between absorbed dose and fluorescence intensity, FAM-DNA-TAMRA （Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine） aqueous solution of different concentrations combined with X-ray irradiation was used to measure the fluorescence intensity. The results showed that the linear relation between fluorescence intensity and absorbed dose was good for different-concentration FAM-DNA-TAMRA aqueous solution containing fluorescent probe in the dose range of 0-20 Gy. The fluorescence had good stability, but the fluorescence background affected the sensitivity of absorbed dose greatly. The results suggest that the FAM-DNA-TAMRA fluorescent probe can be used to measure the absorbed dose of aqueous solution.