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RPS27L基因作为辐射损伤标志物的研究

Study on RPS27L gene as a marker of radiation damage
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摘要 以人外周血为研究对象,研究照射后RPS27L基因的时间效应和剂量效应以及本底水平,探究其作为辐射损伤早期生物标志物的可行性。采集3名健康成人外周血,利用γ射线对其进行照射至吸收剂量分别为0、0.5、1、2、4、6和10 Gy,受照后37℃培养2、4、8、12和24 h,利用实时荧光定量PCR检测RPS27L基因的表达变化,并分析在37个健康人个体中的本底水平。结果显示,受照后的4、8、12和24 h RPS27L基因表达变化显著,而且具有很好的剂量依赖关系;在不同年龄和性别的个体中RPS27L本底水平相对稳定。因此,RPS27L基因具有作为新的辐射损伤标志物的潜力。 In order to explore RPS27L＇s possibility as a biomarker of radiation damage, we studied its time-response and dose-response after irradiation as well as its background level. Three healthy adults＇ peripheral blood was exposed to gamma-rays with 0, 0.5, 1, 2, 4, 6, and 10 Gy, separately and cultured for 2, 4, 8, 12, and 24 h respectively. RPS27L＇s expression change was detected by quantitative real-time PCR. We also analyzed its background level in 37 individuals. The results showed that RPS27L had a significant difference at 4, 8, 12, 24 h and a great dose-independent response. There was also a uniform background level of RPS27L among different age and gender. Thus, RPS27L is of great potential to be a new biomarker of radiation.
作者 李孝鑫 扈江伟 张学清 沈丽萍 李琳 常诚 王治东 陈英
机构地区 安徽医科大学 军事医学科学院放射与辐射医学研究所 解放军
出处 《辐射研究与辐射工艺学报》 CAS CSCD 2015年第5期30-36,共7页 Journal of Radiation Research and Radiation Processing
基金 全军医学科技"十二五"科研项目(BWS11J018) 全军后勤科研重大项目(AWS14C014) 解放军307医院院创新基金(JS-2014-04)项目资助
关键词 核辐射 RPS27L 基因表达变化 实时荧光定量PCR 生物标志物 Nuclear radiation, RPS27L, Gene expression change, Quantitative real-time PCR, Biomarker
分类号 TL71 [核科学技术—辐射防护及环境保护]
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参考文献19

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辐射研究与辐射工艺学报
2015年 第5期

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