ER、PR、p53和HER2在正常子宫内膜和子宫内膜癌中的表达相关性分析

Analysis of corelation between ER PR P53 and HER2 expression in normal endometrium and endometrial carcinoma

目的研究ER、PR、p53和HER2在正常子宫内膜和子宫内膜癌中的表达。方法分别用HE染色和免疫组化SP法,对91例未接受过放、化疗及性激素治疗的子宫内膜癌患者标本,30例正常增生期子宫内膜标本进行染色,观察ER、PR、P53和HER2二者中的表达,并对这两种方法染色阳性表达进行比较。结果本研究中正常子宫内膜组织的30例,ER、PR阳性表达率分别为100%、90.0%,二者的双阳性率为90.0%,91例子宫内膜癌中,二者的双阳性率为47.2%。同时,ER在正常子宫内膜组织的阳性表达率为100%,ER在子宫内膜癌组织的阳性表达率为52.7%,提示与正常组比较,ER在子宫内膜癌组中表达减少,对实验数据进行量化分析表明:其差异有统计学意义(χ2=21.99,P<0.05,表2);PR在正常子宫内膜组织的阳性表达率为90.0%,PR在子宫内膜癌组织的阳性表达率为56.0%,提示与正常组比较,PR在子宫内膜癌组表达减少,对实验数据进行量化分析表明:其差异有统计学意义(χ2=11.36,P<0.05)。结论 ER、PR在子宫内膜癌组中蛋白表达降低。p53在子宫内膜癌组中的高表达,且其差异具有统计学意义(χ2=30.24,P<0.05)。HER2在子宫内膜癌组中的高表达,且其差异具有统计学意义(χ2=30.24,P<0.05)。

Objective To study the expression of ER,PR,P53 and HER2 in normal endometrium and endometrial carcinoma. Methods Using HE staining and immunohistochemistry of SP method,91 cases had not received,chemotherapy and hormone therapy of endometrial carcinoma,30 cases of normal proliferative endometrial samples were stained to observed the expression of Er,PR,p53,HER2 two,and of the two methods dye color positive expression were compared. Results Of this study in normal endometrial tissues of 30 cases,ER and PR positive expression rate of 100%,90. 0%,respectively,two double positive rate was 90. 0%,91 cases of endometrial carcinoma,two double positive rate was 47. 2%. At the same time,the positive expression of ER in normal endometrium was 100%,the positive expression of ER inendometrial carcinoma was 52. 7%,compared with the normal group,the reduction of the expression of ER in endometrial carcinoma group,the experimental data for quantitative analysis showed that： the difference was statistically significant（ χ2= 21. 99,P〈0. 05,table 2）; the positive expression of PR in normal endometrium was 90%,the positive expression of PR inendometrial carcinoma was 56%,compared with the normal group,the reduction of the expression of PR in endometrial carcinoma group,the experimental data for quantitative analysis showed that there were statistically significant differences between them（ χ2= 11. 36,P〈0. 05）. Conclusions ER,PR protein in endometrial carcinoma group decreased expression. The high expression of p53 in endometrial carcinoma group,and the difference was significant（ χ2= 30. 24,P〈0. 05）. The high expression of HER2 in endometrial carcinoma group,and the difference was significant（ χ2= 30. 24,P〈0. 05）.