氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究

In Vitro Effects of Mercury on Steroid Hormone Biosynthesis of Leydig Cells

[目的]探讨氯化汞(Hg Cl2)对SD大鼠体外培养睾丸间质细胞(leydig cell)类固醇激素合成的影响。[方法]不同浓度Hg Cl2(1×10-8、1×10-7、1×10-6、1×10-5 mol/L)作用于leydig细胞后,测定孕酮含量,运用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测Hg Cl2对leydig细胞中St AR、P450scc和3β-HSD m RNA表达的影响。[结果]经不同浓度Hg Cl2染毒后,leydig细胞分泌孕酮的量均有所降低,其中1×10-7、1×10-6、1×10-5 mol/L Hg Cl2染毒组与对照组相比,差异均有统计学意义(P<0.05)。1×10-7、1×10-6和1×10-5 mol/L Hg Cl2剂量组的St AR、P450scc和3β-HSD m RNA相对表达量均低于对照组(P<0.05)。[结论]汞可以影响睾丸间质细胞类固醇激素的合成。

[ Objective] To assess the effects of mercury chloride （HgC12） on steroid hormone biosynthesis in leydig cells isolated from SD rat testis. [ Methods ] Leydig cells were exposed to various concentrations of HgCl2 （1 × 10^8, 1 ×10^-7, 1 × 10^-6, and 1 × 10^-5 mol/L）. Progesterone in the supernatant was measured. The levels of mRNA expression of StAR, P450scc, and 3β-HSD in leydig cells were determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction （RT-PCR）. [ Results ] The progesterone content secreted by Leydis cells was reduced after treated by various concentrations of HgC12, and the progesterone contents in the 1 × 10^-7, 1 ×10^-6, and 1× 10^-5 mol/L HgCl2 groups were significantly lower than that of the control group （P〈0.05）. The mRNA relative expression levels of STAR, P450scc, and 3β-HSD in the leydig cells exposed to HgCl2 at the concentration of 1 × 10^-7, 1×10^-6, and 1 × 10^-5 mol/L were also statistically lower than that of the control group（P〈0.05）. [ Conclusion ] Mercury could affect steroid hormone biosynthesis of leydig cells.