摘要
目的 通过观察免疫性血小板减少症(ITP)患者外周血中辅助性T(Th)细胞相关细胞因子水平的变化,探讨其与ITP发病的关系.方法 应用微量样本多重蛋白定量技术(CBA)检测和比较35例慢性ITP患者和30例健康体检者(正常对照组)外周血中IL-2、IL-4、IL-6、IFN-γ和IL-17A的水平.结果 ITP患者外周血IL-17A水平较正常对照组显著升高,两组IL-17A的水平分别为(6.767±1.066)pg/ml和(1.679±0.253)pg/ml,比较有统计学差异(P<0.01);而患者外周血IL-2、IL-4、IL-6、IFN-γ水平与正常对照组相比均无统计学差异(均P >0.05).血小板计数≥20×10^9/L的患者外周血IL-17A水平为(8.217±1.477)pg/ml,血小板计数< 20×10^9/L的患者外周血IL-17A水平为(5.238±1.490)pg/ml,两者间比较无统计学差异(P>0.05).结论 慢性ITP患者外周血IL-17A水平异常升高,提示Th17细胞和IL-17A可能在ITP的发生、发展中发挥重要的作用.
Objective To investigate the levels of T helper cell-related cytokines in peripheral blood of patients with immune thrombocytopenia (ITP). Methods The levels of IL-2, IL-4, IL-6, IFN-γ and IL-17A in peripheral blood of 35 patients with chronic ITP and 30 healthy subjects (control group) were detected with cytometric bead array(CBA) method. Results The level of IL-17A in peripheral blood of patients with ITP was significantly higher than that of control group [(6.767±1.066)pg/ml vs (1.679±0.253)pg/ml, P〈0.01]; while there were no significant differences in the levels of IL-2, IL-4, IL-6 and IFN-γ in peripheral blood between two groups(P 〉0.05). There were no significant differences in IL-17A levels between patients with PLT≥20×10^9/L and those with PLT〈20×10^9/L [(8.217±1.477)pg/ml vs (5.238±1.490)pg/ml, P 〉0.05], Conclusion Increased IL-17A levels in peripheral blood are detected in patients with chronic ITP.
出处
《浙江医学》
CAS
2015年第18期1526-1528,共3页
Zhejiang Medical Journal
基金
浙江省绍兴县科技项目(201201)