摘要
目的研究大鼠应激性心肌病模型心肌组织中脑钠肽(BNP)的表达变化,探讨其在应激性心肌病导致心源性猝死法医诊断中的应用价值。方法采用制动加电击的急性复合应激方法,建立大鼠应激性心肌病模型。采用酶联免疫吸附试验(Elisa)检测大鼠心肌中BNP的浓度,免疫组织化学和图像分析法检测大鼠心肌BNP表达和光密度平均值,RT-PCR法检测大鼠心肌组织中BNP mRNA的表达变化。结果实验组大鼠血清BNP浓度(95.088±9.925)明显高于正常组(60.825±7.829),差异有统计学意义(P<0.01);免疫组化示心肌细胞胞浆阳性着色,半定量分析结果显示实验组蛋白平均光密度(0.169±0.279)高于对照组(0.144±0.572)差异有统计学意义(P<0.01);RT-PCR检测结果显示实验组心肌组织BNP mRNA的表达量(-2.058±0.909)与对照组(-4.895±1.116)差异明显(P<0.001)。结论应激情况下心肌组织中BNP蛋白及BNP mRNA的表达明显增高,可辅助应激性心肌病的诊断,为相关鉴定提供参考和依据。
Objective This research was aimed to investigate the expression of brain natriuretic peptide（ BNP） in myocardial tissues of rat models and to explore the role of BNP in diagnosis of SCD result from Stress Cardiomyopathy. Methods Rat models of stress cardiomyopathy were established by immobilization stress combined with electric shock. Serum BNP concentrations were detected by Elisa method. The expression of BNP protein in myocardial tissue after stress cardiomyopathy was detected by immtmohistochemistry and the mean density was analyzed by image analysis. Meanwhile,the expression of BNP mRNA was detected by real-time RT-PCR. Results The expression of BNP in serum was significantly higher（ 95. 088 ± 9. 925） in model group than in control group（ 60. 825 ± 7. 829）,there were statistical differences between the two groups（ P〈0. 001）. Immunohistochemistry shows that positive staining of BNP in myocardial cytoplasm.According to semiquantitative analysis,the expression of BNP protein（ 0. 169 ± 0. 279） is higher in models than in the control group（ 0. 144 ± 0. 572）（ P〈0. 05）. The expression of BNP mRNA of model group（-2. 054 ±0. 909） increased,there were statistical differences too（ P〈0. 001）. Conclusion The expression of BNP protein and BNP mRNA were obviously higher in myocardial tissue in condition of stress which maybe play an important role in the diagnosis of stress cardiomyopathy.
出处
《中国法医学杂志》
CSCD
2015年第5期459-462,467,共5页
Chinese Journal of Forensic Medicine
