实脾固肾化瘀方对阿霉素肾纤维化大鼠肾功能保护作用的研究

Research on Renal Function Protective Effects of Shipi Gushen Huayu Recipe in Rats with Adriamycin Induced Renal Fibrosis

目的:探讨实脾固肾化瘀方对阿霉素(ADR)肾纤维化大鼠肾功能的保护作用及其可能的机制。方法:将雄性SD大鼠40只随机分为4组(正常组、模型组、治疗组、对照组),除正常组外,其余大鼠均采用尾静脉注射阿霉素(4 mg/kg)法制成ADR肾纤维化大鼠模型。造模后2周,治疗组予实脾固肾化瘀方浸膏(43 g/kg)灌胃;对照组予福辛普利钠溶液(2 mg/kg)灌胃;正常组和模型组予生理盐水灌胃,1/d。观察30 d后留取大鼠24 h尿蛋白定量,取血处死并取肾脏组织,观察各组Ⅰ型胶原(ColⅠ)、层黏蛋白(LN)、转化生长因子-β1(TGF-β1)及α-平滑肌肌动蛋白(α-SMA)表达变化。结果:治疗组大鼠肾组织ColⅠ、LN、TGF-β1及α-SMA表达较模型组明显下降(P<0.01),与对照组比较差异无统计学意义(P>0.05)。结论:实脾固肾化瘀方能显著改善ADR肾病大鼠肾纤维化,保护肾功能,减轻肾功能损害,与福辛普利钠比较,前者更具有优势。

Objective： To observe renal function protective effects of Shipi Gushen Huayu Recipe on adriamycin（ ADR） induced renal fibrosis in rats and its probable mechanism. Methods： 40 male SD rats were randomly divided into four groups（ normal group,model group,treatment group and control group）,in addition to normal group,the rest rats were created to renal fibrosis model with adriamycin（ 4 mg / kg） by tail intravenous injection. Treatment group were administered by Shipi Gushen Huayu Recipe extract（ 43 g/kg） after creation of model for 2 w; Control group were administered by fosinopril solution（ 2 mg/kg）; Both normal group and model group were administered by saline one time a day. After 30 days,all rats were examined 24 hours urine protein quantity,blood were collected before sacrificed,and kidney tissue were remained to observe the expression of Col Ⅰ（ Collagen Ⅰ）,LN（ Laminin）,TGF- β1（ Transfer growth factor- β1） and α- SMA（ α- Smooth muscle albumin） in treatment group. Results： Expression of ColⅠ,LN,TGF- β1and α- SMA in treatment group were significantly decreased than that in model group（ P〈0. 01）. There was no statistically significant difference compared with control group（ P〉0. 05）. Glomerular mesangial cells and basement membrane hyperplasia in treatment group were improved significantly than that in model group under light microscope. Conclusion： Shipi Gushen Huayu Recipe can significantly improve ADR induced renal fibrosis in rats,alleviate renal function impairment,compared to fosinopril sodium the former has more advantages.