AEG-1和NF-kB p65在人肝细胞癌中的表达及其临床意义

Clinical significance of expression of AEG-1 and NF-?B p65 proteins in human primary hepatocellular carcinoma

目的：探讨人肝细胞癌（hepatocellular carcinoma,HCC）组织中星形细胞上调基因-1（astrocyte elevated gene-1,AEG-1）、核因子-κB（nuclear factor-κB,NF-κB）p65的表达及临床意义.方法：采用免疫组织化学SP法检测AEG-1和NF-κB p65蛋白在40例HCC组织、对应癌旁肝组织及8例正常肝组织中的表达情况.用Western blot方法检测肝癌及癌旁肝组织、正常肝组织中AEG-1和NF-κB p65的表达水平.应用Kaplan-Meier法分析AEG-1和NF-κB p65的表达与肝癌患者预后的关系.结果：HCC组织、癌旁肝组织、正常肝组织中的AEG-1阳性表达率分别是72.5%（29/40）、60%（24/40）、12.5%（1/8）,三者之间的差异具有统计学意义（χ^2=9.74,P〈0.05）.且AEG-1在HCC组织、癌旁组织中的表达明显高于在正常肝组织中的表达（P〈0.05）.NF-κB p65在HCC组织、癌旁组织、正常肝组织中的阳性表达率分别是75%（30/40）、62.5%（25/40）、12.5%（1/8）,三者之间的差异具有统计学意义（χ^2=11.29,P〈0.05）,且NF-κB p65在HCC组织、癌旁组织中的表达明显高于在正常肝组织中的表达（P〈0.05）.Western blot结果与免疫组织化学结果一致.AEG-1、NF-κB p65双阳性组的生存率低于单阳性组,差异有显著性（P〈0.05）.结论：AEG-1可能通过上调NF-κB p65的表达而促进HCC的发生和转移,联合检测AEG-1及NF-κB p65在人肝细胞癌中的表达,有望成为肝癌分子靶向治疗及预后评价的重要指标.

AIM：To detect the expression of astrocyte elevated gene-1（AEG-1） and nuclear factorkB（NF-kB） p65 proteins in hepatocellular carcinoma（HCC） and to analyze their clinical significance.METHODS：The expression of AEG-1 and NFkB p65 proteins was detected by immunohistochemical analysis in 40 HCC samples,40 tumor adjacent tissue samples,and 8 normal hepatic tissue samples.Western blot was also performed for detecting protein levels of AEG-1 and NF-kB p65 in part of the above tissue samples.The relationship between survival and expression of AEG-1 and NF-kB p65 was analyzed by Kaplan-Meier analysis.RESULTS：The positive expression rates of AEG-1 in HCC,tumor adjacent tissue and normal tissue samples were 72.5%（29/ 40）,60%（24/ 40）,and 12.5%（1/ 8）,respectively;the differences among the three groups were statistically significant（χ^2 = 9.74,P〈0.05）.The expression of AEG-1 protein was significantly higher in HCC and tumor adjacent tissue than in normal tissue（P〈0.05）.The expression rates of NF-κB p65 protein in HCC,tumor adjacent tissue and normal tissue samples were 75%（30/ 40）,62.5%（25/ 40）,and 12.5%（1/ 8）,respectively;the differences among the three groups were statistically significant（χ^2 =11.29,P〈0.05）.The expression of NF-kB p65 protein was significantly higher in HCC and tumor adjacent tissue than in normal tissue（P〈0.05）.The expression of AEG-1 and NFkB p65 protein determined by Western blot was similar to that by immunohistochemical analysis.The survival rates in patients with positive expression of AEG-1 and NF-kB p65 were significantly lower than those in patients with negative expression（P〈0.05）.CONCLUSION：AEG-1 may promote invasion and metastasis of HCC through up-regulation of NF-kB p65 expression and activity.The combined detection of expression of AEG-1and NF-kB p65 has significance in gene therapy and in predicting the prognosis of patients with HCC.