白萝卜提取物对不完全性肠梗阻大鼠小肠黏膜过氧化损伤的保护作用

Protective effects of Raphanus sativus extract on intestinal mucosal peroxide damage in rats with incomplete intestinal obstruction

目的:建立大鼠回肠不完全性肠梗阻(incomplete intestinal obstruction,ⅡO),探究白萝卜提取物(Raphanus sativus extract,Rex)对梗阻所致肠黏膜过氧化损伤的保护作用.方法:SD大鼠随机分为正常组(A组,n=14)、假手术组(B组,n=14)、模型组(C组,n=20)和治疗组(D组,n=20).采用末端回肠套环法建立ⅡO大鼠模型,造模成功后,每天D组灌胃Rex药汁(100 mg/kg),其余各组灌胃等量生理盐水,并分别于第5天、7天开腹分离腹主动脉,取血3 mL用于超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)血清学测定,取梗阻部位以上回肠组织4 cm行苏木精-伊红(HE)染色,同时制备组织匀浆用于检测二胺氧化酶(diamine oxidase,DAO).结果:与B组比较,5、7d时,C组SOD活性和DAO含量均降低(P<0.05或P<0.01),而MDA含量均显著升高(P<0.01),肠钻膜损伤评分显著增加(P<0.01),出现肠钻膜上皮细胞肿胀,偶有坏死,脱落,黏膜下层、肌层及浆膜层明显瘀血,并伴有炎细胞浸润;与C组比较,D组给药Rex后,SOD活性升高(P<0.05或P<0.01),DAO含量显著增加(P<0.01),MDA含量显著降低(P<0.01),黏膜损伤评分明显降低(P<0.01),回肠钻膜破损,炎细胞浸润及血管反应均明显减轻.结论:Rex可以提高SOD活性,降低MDA含量,同时增加肠组织内DAO含量来减轻IIO对大鼠肠黏膜的过氧化损伤,对肠黏膜具有保护作用.

AIM：To investigate the protective effects of Raphanus sativus extract（Rex） on intestinal mucosal peroxide damage in a rat model of incomplete intestinal obstruction（ⅡO）.METHODS：Sprague-Dawley rats were randomly divided into a normal group（group A,n =14）,a sham-operation group（group B,n =14）,an intestinal obstruction group（group C,n = 20） and an intestinal obstruction ＋ Rex group（group D,n = 20）.ⅡO was induced by surrounding the terminal ileum with a sterile pipe.After that,the animals in group D was given Rex（100 mg/ kg body weight）,while the rest groups were given equal volumes of saline.On days 5 and 7,the abdominal cavity was opened to collect 3 mL blood from the abdominal aorta for determining superoxide dismutase（SOD） and malondialdehyde（MDA） levels.Meanwhile,two segments of the small intestine were taken 4 cm above the obstructive for hematoxylin-eosin（HE）staining and for measuring diamine oxidase（DAO）,respectively.RESULTS：At each corresponding time point,compared with group B,SOD and DAO levels were significantly lower（P〈0.05 or P〈0.01）and MDA content and mucosal injury score were significantly higher（P〈0.01） in group C.Swelling,necrosis and shedding were visible in epithelial cells.Meanwhile,the submucosa,muscular and serosa had apparent congestion with infiltration of inflammatory cells.Compared with group C,treatment with Rex in group D significantly increased SOD activity（P〈0.05 or P〈0.01） and DAO content（P〈0.01）,while MDA content and mucosal injury score were significantly decreased（P〈0.01）.The damage of the ileum mucosa,infiltration of inflammatory cells and vascular responses in the ileum were significantly alleviated after treatment with Rex.CONCLUSION：Rex can elevate serum SOD activity,reduce MDA content and increase tissue DAO levels in rats with IIO to protect the intestinal mucosal from being damaged by oxidative stress reaction effectively.