脐带间充质干细胞移植对老年痴呆小鼠SAMP8学习记忆的影响

Effects of HUMSCs on Learning Ability and Memory in Mice SAMP8 with Alzheimer's Disease

目的:探讨脐带间充质干细胞(HUMSCs)移植对老年痴呆小鼠SAMP8学习记忆能力的影响。方法:培养人脐带间充质干细胞,通过尾静脉以高中低3个剂量(2.5×105/0.2 m L/20 g、5×105/0.2 m L/20 g、1×106/0.2 m L/20 g)分别向7月龄SAMP8老年痴呆小鼠移植HUMSCs,并设立移植生理盐水组和R1小鼠正常对照组。HUMSCs移植45 d后,MORRIS水迷宫和跳台实验检测其学习记忆能力。结果:与R1正常对照小鼠比较,SAMP8老年痴呆小鼠生理盐水组学习记忆能力明显降低;和移植生理盐水组比较,细胞移植低剂量组水迷宫逃避潜伏期显著缩短(P<0.05),穿越平台次数显著增加(P<0.05);跳台潜伏期显著延长(P<0.05),错误次数显著减少(P<0.05),结论:低剂量的脐带间充质干细胞移植能增强老年痴呆小鼠SAMP8的学习记忆能力。

Objective：To explore the effects of human umbilical cord mesenchymal stem cells（HUMSCSs） on the learning ability and memory of mice SAMP8 with Alzheimer＇s disease.Methods：We cultivated human umbilical cord mesenchymal stem cells,provided three doses HUMSCs（2.5×10~5/0.2 mL/20 g,5×10~5/0.2 mL/20 g,1×10~6/0.2 mL/20 g） for the transplantation of seven months SAMP8 Alzheimer＇s mice by the tail vein,and set up the normal saline group and normal control group R1 mice for comparison.After 45 days,MORRIS water maze and jumping apparatus were used to test the ability of learning and memory of the subjects.Results：Compared with normal control mice R1,normal saline group of SAMP8 Alzheimer＇s mice performed significantly worse on the learning and memory tests.But there was significant improvement in Morris water maze test compared with the normal saline group,including shorter escape latency（P 0.05） more platform crossing frequencies（P 0.05）,and in jumping apparatus including less jumping stand error number（P 0.05） and longer step down latency（P 0.05）.Conclusion：Low dose of HUMSCs can enhance learning and memory ability of SAMP8 Alzheimer＇s mice.