不同生长方式防风色原酮物质含量测定及差异性分析

Content Determination and Difference Analysis of Chromone in Fangfeng with Different Growth Patterns

目的:以同时测定防风中的两种色原酮物质升麻素苷和5-O-甲基维斯阿米醇苷为目标,建立了上述两种物质的的含量测定方法。方法:色谱柱采用Agilent TC-C18(250 mm×4.6 mm,5μm);梯度洗脱的流动相为水、甲醇,检测波长254nm,流速1 m L/min,柱温35℃,进样量10μL。结果:不同生长方式的防风样品的5-O-甲基维斯阿米醇苷含量无统计学意义,野生和栽培防风的升麻素苷含量有差异,不同生长方式对防风样品的色原酮含量有差异。结论:野生防风质量较好,同时测定两种色原酮方法简便,结果准确,精密度、重现性、稳定性较好,可以此作为防风中升麻素苷和5-O-甲基维斯阿米醇苷的测定方法。

Objective：To establish a content determination method to simultaneously determine two kinds of chromoneS including Prim-O-glucosylcimifugin and 4＇-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng.Methods：Agilent TC-C_（18）（250 mm×4.6 mm,5 μm） was used as chromatographic column;water and methanol were used as gradient elution of the mobile phase,wave length of 254 nm was detected,with the flow rate of 1 mL / min,column temperature of 35℃,the injection volume 10μL.Results：4＇-O-beta-Glucopyranosyl-5-O-Methylvisamminol content of Fangfeng with different growth patterns had no significant difference;there was difference in the content of Prim-O-glucosylcimifugin between wild and cultivated Fangfeng;there were differences in Chromones contents in Fangfeng with different growth patterns.Conclusion：Wild Fangfeng has better quality,and the simultaneous determination of two Chromones method is simple,accurate,with good precision,reproducibility and stability,and this can be used as determination method of Prim-O-glucosylcimifugin and 4＇-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng.