高纯度牛血清白蛋白的研制

THE PREPARATION OF HIGHLY PURIFIED BOVINE SERUM ALBUMIN

作者用辛酸钠—离子交换法从牛血清分离和纯化白蛋白。结果表明:用本法制得的白蛋白纯度高,SDS—PAGE呈现单一染色带,优于国内外同类产品。产品的收率提高约50%。本法操作简便、可用于生产制备。

Albumin from bovi-ne serum was separated and purified bySodium caprylate-Ionexchange chromatog-raphy. The product was analyzed. Theresults showed that UV absorption of theproduct is at 278 nm; Molecular weightis 66.2 Kd and it presented a single bandof coomassie brilliant blue stained onSDS-PAGE. The purity of BSA producedby us is superior to that of domestic or for-eign products. The recovery of the meth-od was also estimated and we consider itto be a simple valid method for mass pr-oduction.