树莓果粉对I型糖尿病大鼠血糖、免疫反应及氧化应激的影响

Effects of Raspberry Pulp Powder on Blood Glucose,Immune Response and Oxidative Stress in Type I Diabetic Rats

本研究采用链脲霉素(STZ)诱导的大鼠模型来研究树莓果粉在Ⅰ型糖尿病中降血糖、免疫调节及抗氧化的活性。结果发现树莓果粉能增加糖尿病大鼠的体重,降低其血糖,并对糖尿病大鼠受损的胰岛有一定的修复作用。同时,树莓果粉能明显降低糖尿病大鼠血清中CD4、CD8分子、干扰素-γ(IFN-γ)、白介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)以及免疫球蛋白Ig G、Ig A和Ig E的含量。此外,树莓果粉能显著增强糖尿病大鼠胰腺和血清中超氧化物歧化酶(SOD)的活性,增加还原型谷胱甘肽(GSH)含量,并明显降低丙二醛(MDA)的含量。说明树莓果粉能通过减缓自身免疫反应程度和缓解氧化应激来改善胰岛β细胞的损伤,从而达到治疗Ⅰ型糖尿病的作用。

In this study, the anti-hyperglycemic, immunoregulation and antioxidant activities of raspberry pulp powder （RPP） on diabetic rats induced by streptozotocin （STZ） were investigated. The results revealed that RPP ameliorated the loss of body weight,lowered the blood glucose,and restored the damaged islet in diabetic rats. It was also found that RPP can effectively decrease the cluster of differentiation 4 （ CD4）, cluster of differentiation 8 （ CD8 ）, tumor necrosis factor-α （TNF-α） ,intefferon-γ （ IFN-γ）, interleukin-2 （ IL-2）, immunoglobulin G （IgG）, immunoglobulin A （IgA） and immunoglobulin E （IgE） in the serum of the diabetic rats. Simultaneously, after RPP treatment, the activity of superoxide dismutase （SOD） and content of reduced glutathione （GSH） were significantly increased,while the content of malonaldehyde （MDA） was markedly decreased in pancreas tissue and serum of STZ-induced diabetic rats. Taken togeth- er, these results suggested that RPP fuiacfioned as an effective anti-diabetes component and it may protect the β-cel/s of the pancreatic islet through attenuating autoimmtmity response and oxidative stress.