响应面优化野生樱桃李叶总黄酮的超声辅助提取工艺

Optimization of Ultrasonic-assisted extraction of Total Flavonoids from Prunus divaricata Ldb Leave Using Response Surface Methodology

以具有地域特色的野生樱桃李叶为实验材料,在单因素试验基础上,以乙醇体积分数、提取温度、料液比为自变量,总黄酮提取量为响应值,利用响应面法优化野生樱桃李叶总黄酮的超声辅助提取最佳条件为乙醇体积分数51%、提取温度72℃、料液比1∶45 g/m L,在300 W功率下超声提取30 min,提取次数2次,总黄酮提取量为38.27 mg/g,达到预测值的98.3%。超声辅助提取较传统索氏提取操作简单,耗时少,提取效率高,是野生樱桃李叶总黄酮提取的一种有效方法。

The optimal conditions for the ultrasonic-assisted extraction of total flavonoids from Prunus divaricata Ldb leave were determined using response surface methodology. Based on single factor experiments, the influence of ethanol concentration,extraction temperature and solid-liquid ratio on the extraction yield of total flavonoids were investigated. The optimal conditions were determined as foUows：ethanol concentration was 51%, extraction temperature was 72℃, solid-liquid ratio was 1：45 （g/mL） ,ultrasonic power was 300 W,for 2 times,each time 30 rain,the yield of total fla- vonoids was 38.27 mg/g,which accounts for 98.3% of the theoretically predicted value. Compared with the traditional Soxhlet extraction, the ultrasonic-assisted extraction was more convenient,less time-consumlng, higher extraction efficiency. Hence,the ultrasonic-assisted extraction can be used as an effective method for the extraction of total flavonoids from P. divaricata leave.