矮垂头菊乙醇提取物的体外自由基清除活性及其对高原缺氧小鼠的保护作用研究

In vitro Free Radical Scavenging Activity of Ethanol Extract of Cremanthodium humile and Its Protective Effect on Mice under Hypobaric Hypoxia Condition

本文对矮垂头菊乙醇提取物(Ethanol extract of Cremanthodium humile,EECH)中总黄酮与总多酚含量,体外自由基清除活性以及对高原缺氧小鼠的保护作用进行研究。结果显示:EECH中总黄酮与总多酚的含量分别为378.35±7.04 mg芦丁/g提取物和114.58±0.99 mg没食子酸/g提取物。其对DPPH、羟自由基、NO自由基均表现出一定的清除活性,IC50值分别为80.90±0.47、263.54±6.82和285.26±4.45μg/m L,但均弱于Vc,然而其对超氧阴离子的清除作用优于Vc。此外,EECH能够显著延长缺氧常压密闭缺氧小鼠的存活时间,减少低压低氧小鼠心脑组织中MDA含量,提高抗氧化酶SOD、CAT和GSH-Px的活力。以上结果表明:EECH是一种活性优异的天然抗氧化剂,对高原缺氧诱导的小鼠心脑组织损伤具有明显的保护作用。

The objective of this study was to investigate the content of total phenolics and total flavonoid and free radical scavenging activity of Cremanthodium humile （EECH） as well as its protective effect against hypobaric hypoxia （HH） induced injury on mice. According to the restrlts, the contents of total flavonoid and total phenolics in EECH were 378.35 ± 7.04 mg Ru/g extract and 114. 58±0, 99 mg GAE/g extract, respectively. EECH showed excellent scavenging effect on DPPH,hydroxyl,nitric oxide radical with the IC50 value of 80.90 ± 0. 47 ,263. 54±＋ 6.82 and 285.26 ± 4, 45 μg/mL, but still weaker than Vc. While EECH exhibited stronger scavenging activity on superexide than Ve. Administration of EECH can prolong the survival time of mice under normobarlc hypoxia, decrease the level of MDA in brain and heart of mice under HH condition and increase the activity of SOD, CAT and GSH-Px. All of these results indicated that EECH could be regarded as an excellent source of natural antioxidants and prevented damage from HH induced brain and heart injury.