（1，3）-β-D葡聚糖检测在深部真菌感染中的应用价值

The detection value of （1,3）-β-D-glucan in clinical deep fungal infection

目的：探讨（1，3）-β-D葡聚糖检测（G试验）在深部真菌感染临床早期诊断中的应用价值。方法选取住院治疗的深部真菌感染患者86例（观察组），另选志愿参与该次研究的健康者24例作为对照组，对两组研究对象行血浆 G 试验，同时培养可疑感染标本，并将深部真菌感染患者按照培养结果分为阴性组及阳性组，对血浆 G 试验结果进行比较分析。结果对照组血浆 G 试验结果为（6．12±2．87）×10－3 ng／mL，深部真菌感染阴性组、阳性组血浆 G 试验结果分别为（32．58±24．12）×10^－3 ng／mL、（101．28±36．04）×10^－3 ng／mL，与对照组比较，深部真菌感染阴性组、阳性组血浆 G 试验结果均显著升高，差异均有统计学意义（t ＝18．28、7．92，P ＝0．036、0．042）；同时阳性组血浆 G 试验结果显著高于阴性组，差异有统计学意义（t ＝12．16， P ＝0．038）。血浆 G 试验诊断深部真菌感染阳性率为79．1％，明显高于真菌培养的50．0％，差异有统计学意义（χ^2＝31．26，P ＝0．002）。结论在深部真菌感染的早期临床诊断中，血浆 G 试验检测阳性率较高，优于真菌培养，值得临床中广泛应用。

Objective To discuss the detection value of（1,3）-β-D -glucan in the early diagnosis of fun-gal infection in clinic.Methods 86 patients with deep fungal infection were selected as the study group,and 24 cases of healthy volunteers were selected as the control group,all groups took plasma G test,while cultivating specimens sus-pected infection and deep fungal infections in patients were divided according to culture -negative group and positive group,plasma G test results were compared.Results In the control group,G test was （6.12 ±2.87）×10 -3 ng/mL in plasma,deep fungal infection negative,positive test results were （32.58 ±24.12）×10 -3 ng/mL and （101.28 ± 36.04）×10 -3 ng/mL in plasma G respectively,positive group G results in plasma were significantly increased,the differences were statistically significant（t =18.28,7.92,P =0.036,0.042）;both positive plasma G test results were significantly higher than that of the negative group,the difference was statistically significant（t =12.16,P =0.038）. Plasma G test positive diagnosis of deep fungal infection was 79.1%,significantly better than the fungal culture 50.0%,the difference was statistically significant（χ2 =31.26,P =0.002）.Conclusion In early clinical diagnosis of deep true infection,plasma G test positive rate is higher than fungal culture,and is worthy of wider application.