再生障碍性贫血患者血清白介素17、白介素18及白介素18结合蛋白水平变化及其临床意义

Changes and clinical significance of serum interleukin 17,interleukin 18 and interleukin 18 binding protein in patients with aplastic anemia

目的：探讨血清白介素17（IL-17）、白介素18（IL-18）及白介素18结合蛋白（IL-18BP）水平在再生障碍性贫血患者中的变化及其临床意义。方法应用酶联免疫吸附实验（ELISA）法检测52例再生障碍性贫血患者血清 IL-17、IL-18及 IL-18BP 含量，并与52例对照组比较分析。结果再生障碍性贫血组血清 IL-17、IL-18及 IL-18BP 水平分别为（216．41±22．24）μg／L、（402．37±59．76）ng／L、（8．79±2．61）μg／L，均高于对照组的（90．36±9．78）μg／L、（136．48±20．25）ng／L、（2．01±0．68）μg／L（t ＝37．41、30．46、18．13，均 P ＜0．01）；急性重型再生障碍性贫血（AAA）组 IL-17、IL-18和 IL-18BP 的水平分别为（267．76±30．17）μg／L、（479．41±68．76）ng／L、（11．72±3．29）μg／L，高于慢性再生障碍性贫血（CAA）组的（156．48±18．36）μg／L、（312．47±40．61）ng／L、（5．36±1．45）μg／L（t ＝15．73、10．43、8．92，均 P ＜0．01）；血清 IL-17水平与血清 IL-18及 IL-18BP 水平呈正相关（r ＝0．527，r ＝0．489；均 P ＜0．05），IL-18与 IL-18BP 也呈显著正相关（r ＝0．731，P ＜0．01）。结论IL-17、IL-18和 IL-18BP 共同参与再生障碍性贫血免疫致病过程，且存在一定的相关性，IL-17、IL-18及 IL-18BP 可能影响再生障碍性贫血患者的疾病进程。

Objective To explore the clinical significance of changes of serum.interleukin 17（IL -17）, interleukin 18（IL -18）and interleukin 18 binding protein（IL -18BP）in patients with AA.Methods Serum IL -17,IL -18 and IL -18BP levels were measured by ELISA in 50 patients with AA and 52 health control.Results The levels of serum IL -17,IL -18 and IL -18BP in the AA group were （216.41 ±22.24）μg/L,（402.37 ± 59.76）ng/L and （8.79 ±2.61 ）μg/L respectively,which were higher than those in the healthy control group of （90.36 ±9.78）μg/L,（136.48 ±20.25）ng/L and （2.01 ±0.68）μg/L（t =37.41,30.46,18.13,all P 〈0.01）. And the levels of serum IL -17,IL -18 and IL -18BP in AAA group were （267.76 ±30.17）μg/L,（479.41 ± 68.76）ng/Land （11.72 ±3.29）μg/L,which were higher than those in the CAA group of （156.48 ±18.36）μg/L, （312.47 ±40.61）ng/L and （5.36 ±1.45）μg/L （t =15.73,10.43,8.92,all P 〈0.01）.The serum level of IL -17 was positive correlation with the levels of IL -18 and IL -18BP in patients with AA （r =0.527,r =0.489,all P 〈0.05）,the serum level of IL -18 was also positive correlation with the level of IL -18BP（r =0.731,P 〈0.01）. Conclusion IL -17,IL -18 and IL -18BP may participate in the pathogenesis of AA,and there is a certain correla-tion,which may influence the process of AA.