ICUs分离的三种重要病原菌的耐药特性与基因型分析

Drug Resistance and Homological Analysis of Important Clinical Bacterial Pathogens

目的 了解西京医院重症监护室分离的3种重要感染病原菌的耐药性和同源性.方法 于2013年11月～2014年3月收集西京医院重症监护室分离到的15株金黄色葡萄球菌,24株鲍曼不动杆菌和22株铜绿假单胞菌,应用VITEK-32微生物鉴定药敏分析系统对感染病原菌进行菌种鉴定和药物敏感性试验;应用Diversilab基因分型系统对感染病原菌进行同源性分析.结果 15株金黄色葡萄球菌分为A1,A2,B,C等4型;24株鲍曼不动杆菌分为A和C型两型.22株铜绿假单胞菌分为A1,A2,A3,A4,A5,B,C型等7型.相同菌株不同基因型之间存在不同的耐药性;来自不同区域的同型菌株存在不同的耐药性.结论 同源区域明显相关,综合分析有助于了解院内感染病原菌的流行趋势和感控效果.

Objective To investigate the antimierobial resistance and homology of the 3 clinical bacterial pathogens from the intensive care units of Xijing Hospital. Methods Collected the clinical infectious pathogens,including 15 Staphylococcus aureus ,24 Bauman acinetobacter and 22 Pseudomonas aeruginosa from November 2013 to March 2014. Pathogens were identi- fied with VITEK-32 automatic microorganism analyzer. At the same time, the drug susceptibility testing was performed. The homology was identified by using automated DiversiLab System DNA fingerprinting. Results 15 strains of Staphylococcus aureus could be divided into A1,A2,B and C genotypes. 24 strains of Bauman acinetobacter could be divided into A and C genotypes. 22 strains of Pseudomonas aeruginosa could be divided into A1, A2, A3, A4, A5, B, and C genotypes： There was different resistance among the same strains of different genotypes,and the same genotype strains from different areas had different drug resistance. Conclusion There was a high relationship between the drug resistance, genotype and the area it was from. It was suggested that combined analysis these 3 factors might be helpful for full understand the control of the hos- pital infection.