72例老年急性白血病免疫表型分析

Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia

目的 探讨老年急性白血病流式细胞术免疫分型的特点及临床意义.方法 回顾性分析2010年1月～2015年3月,利用三色法流式分析法检测76例老年性白血病的免疫表型及其特点.结果 ①AML中最主要表达的抗原是MPO,CD13和CD33,表达率分别为98.2％,98.2％和7.2％.47.3％有淋系抗原表达,其中CD7（19.3％）,CD56（26.3％）最为常见.②老年B-ALL中,CD19的表达阳性率最高为100％,其次是cCD79a,CD10和CD20,表达阳性率分别为88.9％,46.2％和66.7％.纯B系抗原表达者77.8％,伴T系抗原表达者22.3％,髓系相关抗原CD13,CD33的表达阳性率分别为33.4％和22.3％.③所有老年T-ALL中均表达CD3与CD7,CD5（＋）细胞占66.7％,干祖细胞CD34,HLA-DR阳性率分别为33.3％和0％.结论 流式细胞术免疫表型分析是一种简便、快速、准确的检测技术手段,结合传统的检测手段,有助于老年白血病的精确诊断.

Objective To explore the flow cytometry（FCM） immune classification of elderly acute leukemic characteristics and clinical significance. Methods Analysed retrospectively the immunophenotype and the character of 76 patients with eld- erly acute leukemia,that performed by 3-color flow cytornetric analysis method. Results ①）The major marker in AML were MPO, CD13, CD33, expression rate of 98.2 %, 98.2 % and 77.2 %, respectively. ② The lymphoid immunophenotypy marker was 47.3 % in all patients, including CD7 （19.3 %）, CD56 （26.3 %. CD19 expression in all older B-lymphoid leukemia, fol lowed by eCD79a,CD10 and CD20 expression positive rate were 88.9% ,46.2% and 66.7% respectively. Pure B antigen ex- pression was 77.8 % ,with T antigen reaches 22.3 %, The expression of myeloid associated antigen CD13 was 33.4 %, and CD33 positive rate was 22.3 %. ③The T-cell special marker CD3 and CD7 were in whole T-ALL, CD5 expression positive rate was 66.7 %. Only 33.3 % cases expressed progenitor cell marker CD34,no had HLA-DR expression. Conclusion FCM immune phenotype analysis is a simple,rapid and accurate means of detection technology, combined with the traditional de- tection methods,will effeeted help accurate diagnosis of senile leukemia.