IL-12诱导的CIK细胞联合化疗治疗食管癌的疗效及对患者免疫功能的影响

IL-12 induced CIK cells combined with chemotherapy improve immune function and efficacy in patients with esophageal carcinoma

目的:探讨白介素(interleukin,IL)-12诱导的细胞因子诱导的杀伤细胞(cytokine-induced killer,CIK)联合化疗治疗食管癌的疗效.方法:将无锡市第三人民医院收治的200例食管癌患者按照随机分组的方法分为对照组和研究组,每组患者100例.两组患者均采用自体CIK细胞输注与奥沙利铂联合方案治疗,自体CIK细胞在体外培养期间,以干扰素-g(interferon,IFN-g)、白细胞分化抗原-3抗体(cluster of differentiation-3 mice antibody,C D3m A b)、I L-2、I L-1a作为诱导剂,研究组患者自体CIK细胞在体外培养期间,以IL-12、IFN-g、CD3m Ab、IL-2、IL-1a作为诱导剂.在经过治疗后检测两组患者临床疗效,外周血T细胞亚群表达情况和CIK体外培养期间的CIK表型分布情况和杀伤活性比较.结果:研究组患者治疗完全缓解、部分缓解以及治疗有效人数及所占比例显著高于对照组(P<0.05),稳定患者数两组患者无显著差异.治疗后疾病进展人数,对照组患者显著高于研究组(P<0.05).治疗后研究组患者外周血CD3+、CD3+C D8+、CD3-C D16+C D56+、CD3+C D16+C D56+T细胞所占比例显著高于对照组患者(P<0.05);研究组患者外周血CD3+C D4+T细胞所占比例和C D3+C D4+/C D3+C D8+显著低于对照组患者(P<0.05).研究组患者CIK细胞CD3+CD4+、CD 3+CD 16+CD 56+T细胞所占比例及C D3+C D4+/C D3+C D8+显著低于对照组,而C D3+C D8+T细胞所占比例则显著高于对照组患者(P<0.05).研究组患者CIK细胞在体外杀伤活性也显著高于对照组患者CIK细胞(P<0.05).结论:IL-12诱导的CIK细胞联合化疗对于食管癌疗效较好,值得临床推广.

AIM：To evaluate the clinical effects of interleukin-12（IL-12） induced killer cytokineinduced（CIK） combined with chemotherapy in patients with esophageal carcinoma.METHODS：Two hundred esophageal cancer patients treated at our hospital were randomly divided into a control group and a study group.All patients were treated with autologous CIK cell infusion and oxaliplatin.The CIK cells of the control group were induced with IFN-y,cd3 mab,IL-2,and IL-1 in in vitro culture period,while those of the study group were induced with IL-12 IFN-y,CD3 mAb,IL-2,and IL-1.After treatment,the clinical effects,the expression of T lymphocyte subsets,the CIK phenotype distribution and killing activity of CIK were compared between the two groups.RESULTS：The numbers of patients who achieved complete remission,partial remission and improvement in the study group were significantly more than those of the control group（P〈0.05）;there was no significant difference in the number of patients with stable disease（P〈0.05）;the number of patients with progressive disease was significantly more in the control group than in the study group（P〈0.05）.After treatment,the percentages of peripheral blood CD3＋,CD3＋CD8＋,CD3-CD16＋CD56＋,and CD3＋CD16＋CD56＋ T cells were significantly higher in the study group than in the control group（P〈0.05）,while that of peripheral blood CD3＋ CD4＋ T cells and the CD3＋CD4＋/CD3＋CD8＋ ratio were significantly lower in the study group（P〈0.05）.The percentages of CD3＋CD4＋ cells and CD3＋CD16＋CD56＋ T cells and CD3＋CD4＋/ CD3＋CD8＋ ratio in CIK cells were significantly lower,and the percentage of CD3＋ CD8＋ T cells was significantly higher in the study group than in the control group（P〈0.05）.The cytotoxic activity of CIK cells in the study group was also significantly higher than that of the control group.CONCLUSION：IL-12 induced CIK cells combined with chemotherapy improve immune function and efficacy in patients with esophageal cancer.