丙型肝炎病毒携带者病毒基因型分布特征及临床意义

Clinical significance and distribution characteristics of hepatitis C virus genotypes in hepatitis C virus carriers

目的:研究丙型肝炎病毒(HCV)携带者病毒基因型流行趋势和分布特征,探讨其临床应用价值。方法:随机收集无临床症状HCV携带者血清样本共计90例,HCV RNA含量大于1×103 IU/ml,其中男58例,女32例。应用聚合酶链式反应(PCR)和反向斑点杂交技术(RDB)进行HCV基因分型检测,并对受检者相关资料进行分析。结果:HCV携带者HCV RNA平均浓度为4.94±1.36lg IU/ml。不同性别组之间HCV RNA含量无明显差异(P>0.05)。所有标本HCV基因成功分型。单基因型感染为主要感染类型,占95.56%(86/90)。其中1b型感染率为48.89%(44/90),2a型感染率为42.22(38/90)。不同性别组HCV携带者HCV基因型分布差异无统计学意义(P>0.05)。结论:本地区HCV携带者感染主要类型为单基因型感染;HCV 1b为主要基因型,其次为2a型;开展HCV基因型检测具有重要的临床意义。

Objective ：To study the prevalence and distribution characteristics of hepatitis C virus （HCV） genotypes and to explore the clinic significance of HCV genotypes in HCV carriers .Methods ：We conducted a cohort study with 90 participants including 58 male patients and 32 female patients .The HCV RNA levels of all sera sam‐ples were confirmed higher than 1 × 103 IU/ml .HCV genotyping was performed using type‐specific primers by means of polymerase chain reaction （PCR） and reverse dot blot （RDB） .The related data of all subjects were ana‐lyzed .GraphPad Prism 5 .0 software was used for statistical analysis .Results ：The average concentration of HCV RNA in sera was 4 .94 ± 1 .36 log IU/ml .All 90 specimens were genotyped successfully .There were no significant difference between the different gender groups about the HCV RNA levels of all sera （P〉0 .05） .HCV 1b was the most predominant genotype 48 .89% （44/90） ,followed by HCV 2a 42 .22% （38/90） .The single HCV genotype in‐fection was frequently detected among all patients .Its infection ratio was 95 .56% （86/90） .There was no significant difference between the different gender groups about the distribution of HCV genotypes（P〉0 .05） .Conclusion ：The single HCV genotype infection is primary infection style .HCV 1b is the most frequently detected genotype in pa‐tients infected with HCV ,following by HCV 2a .It is significant for clinical treatment to detect the genotypes of HCV .