饲粮添加鼠李糖乳酸杆菌GG缓解轮状病毒感染对断奶仔猪血清尿素氮含量和免疫功能的影响

Dietary Lactobacillus rhamnosus GG Supplementation Alleviates the Effect of Rotavirus Infection on Serum Urea Nitrogen Content and Immunity Function of Weaning Piglets

本研究旨在探讨饲粮添加鼠李糖乳酸杆菌GG(LGG)是否可以缓解轮状病毒(RV)感染对断奶仔猪血清尿素氮含量和免疫功能的影响。试验选取24头健康的21日龄断奶的"杜×长×大"仔猪,采用2×2因子试验设计,即LGG处理(添加109CFU/kg LGG饲粮或不添加LGG饲粮)和RV感染(灌服RV或无菌培养液),RV攻毒于试验第15天早上进行。试验期为19 d。结果表明,RV感染显著提高了断奶仔猪料重比(F/G)和血清尿素氮含量(P<0.05),显著降低了血液中CD3+和CD4+T淋巴细胞数量及CD4+/CD8+(P<0.05),显著降低了血清中免疫球蛋白(Ig)G、Ig A和白细胞介素(IL)-4的含量(P<0.05),并显著提高了血清中IL-2和轮状病毒特异性抗体(RV-Ab)的含量(P<0.05)。饲粮添加LGG显著降低了断奶仔猪的F/G和血清尿素氮含量(P<0.05),有提高断奶仔猪血液中CD4+T淋巴细胞数量的趋势(P=0.09),显著提高了血清中Ig G和IL-4的含量(P<0.05)。而与饲喂对照饲粮的RV感染仔猪相比,饲喂添加LGG的饲粮显著降低了RV感染仔猪F/G及血清尿素氮、IL-2含量(P<0.05),显著提高了RV感染仔猪血液中CD4+T淋巴细胞数量和血清IL-4的含量(P<0.05)。综上所述,饲粮中添加LGG可能通过缓解RV感染对免疫功能和体内蛋白质代谢的影响,改善了RV感染仔猪的生长性能。

The experiment was conducted to determine the effect of dietary Lactobacillus rhamnosus GG（ LGG） supplementation on serum urea nitrogen level and immunity function of weaning piglets infected by rotavirus（ RV）. Twenty-four healthy crossbred（ Duroc×Landrace×Yorkshire） piglets weaned at 21 days of age were used in a 2 × 2 factorial design. The main factors consisted of diets（ control diet or supplementing10^9 CFU / kg LGG diet） and RV infection（ oral infusion with RV or sterilized MEMmedium）. RV infection was carried out on day 15. The whole experiment lasted for 19 days. The results showed that RV challenge significantly increased feed / gain（ F / G） and the serum urea nitrogen（ UN） content of weaning piglets（ P〈0. 05）,significantly decreased the numbers of CD3^＋and CD4^＋T lymphocytes and the CD4^＋/ CD8^＋in the blood（ P〈0. 05）,significantly decreased the content of immunoglobulin G（ Ig G）,immunoglobulin A（ Ig A）and interleukin（ IL）-4 in serum（ P〈0. 05）,and significantly increased the content of IL-2 and RV specific antibody in serum（ P〈0. 05）. Dietary LGG supplementation significantly reduced F / G and serum UN content of weaning piglets（ P〈0. 05）,increased the number of CD4^＋T lymphocyte in blood（ P = 0. 09）,and significantly increased the contents of Ig G and IL-4 in serum（ P〈0. 05）. In addition,compared to the RV infected piglets fed the control diet,dietary LGG supplementation significantly decreased the F / G and serum UN content of RV infected piglets（ P〈0. 05）,and significantly increased the number of CD4^＋T lymphocyte of blood and the content of IL-4 in serum（ P〈0. 05）. These results suggest that dietary LGG supplementation can improve the growth performance of weaning piglets infected by RV possibly via alleviating the effect of RV infection on the protein metabolism and the immunity.