泌尿生殖道炎细小脲原体PCR检测结果临床研究

Clinical Research of Detection Results of Ureaplasma Parvun in Urinogenital Tract Infection

目的探讨细小脲原体(Up)在泌尿生殖道炎中的临床特点。方法对1 477例泌尿生殖道分泌物进行Up、沙眼衣原体(Ct)、人乳头状瘤病毒(HPV)、单纯疱疹病毒(HSV)和淋球菌PCR检测和解脲脲原体(Uu)和人型支原体(Mh)培养。1 240例分泌物进行了涂片革兰染色。结果 1 477例泌尿生殖道分泌物检测病原体阳性854例,检出率为57.82%(854/1 477)。Up阳性占26.70%(228/854),其中占男性17.53%(64/365),占女性33.54%(164/489),男女Up阳性率差异有统计学意义。随着分泌物涂片革兰染色分级的增加,泌尿生殖道病原体阳性率也增加,且对比差异有统计学意义,说明分泌物涂片革兰染色对临床感染程度有一定的提示作用。结论 Up是泌尿生殖道炎主要病原体之一。Up感染可见于病人体弱者,在感染病原体量大、感染时间长等条件下可引起临床症状。PCR法检测Up对泌尿生殖道感染高危人群及有临床症状者能够做到诊断准确、快速,有临床应用价值。

Objective To research the clinical features of Ureaplasma parvum（Up） in urinogenital tract infection. Methods The secretion in urinogenital tract was detected by polymerase chain reaction（PCR） examination in Up, Chlamydia trachomatis（Ct）, HPV, HSV and Neisseria gonococcus（NG）, and by Ureaplasma urealytium and Macoplasma homonis culture. Secretion of1 240 cases was smeared with Gram stain. Results 854 cases had positive pathogen by examination of urinogenital tract secretion of 1 477 cases. The pathogen positive rate was 57.82%（854/1477）. The positive rate of Up was 26.70%（228/854）. Of the positive Up cases, male and female positive rates were 17.53%（64/365） and 33.54%（164/489） respectively. The difference of Up positive rates between male and female groups had statistical significance. With the increasing in the smear Gram stain degrees, pathogen positive rates of urinogenital tract also increased, and had statistical significance, showing that the smear Gram stain may suggest inflective degree. Conclusion Up is one of the major pathogens of urinogenital tract infection. Infection caused by Up may be seen in feeble cases and lead to clinical symptoms when quantity of Up is more and continuous infecting time is longer. Detection of Up by PCR may diagnose urinogenital tract infection accurately and quickly in high risk crowd of urinogenital tract infection and with clinical symptoms, and have applicable value in clinic.