熊果酸诱导HL-60细胞凋亡及抑制增殖与PTEN-Akt途径的相关性

Relationship between PTEN-Akt pathway and ursolic acid on apoptosis and in inhibiting the proliferation of HL-60 cells

目的探讨熊果酸诱导人急性髓性白血病细胞系(HL-60)细胞凋亡及抑制增殖作用机制是否与PTEN-Akt信号传导途径相关。方法体外培养HL-60细胞。琼脂糖凝胶电泳观察DNA梯形条带。Western Blot探讨UA对HL-60细胞PTEN、p-Akt蛋白表达的影响。结果 UA可上调HL-60细胞PTEN蛋白表达和抑制P-Akt蛋白表达并呈时间依赖方式,PPARγ特异性阻断剂GW9662预孵育可拮抗此作用。结论熊果酸抑制HL-60细胞增殖及诱导凋亡与其上调PTEN蛋白表达,抑制抑制Akt磷酸化相关。

Objective To investigat the relationship between PTEN-Akt pathway and ursolic acid in induction the apoptosis and in inhibiting the proliferation of HL-60 cells. Methods HL-60 cells line were cultured in vitro. To observe the DNA ladder bands by DNA agarosegel electrophoresis. To explore the influence of UA about the PTEN and p- Akt protein of HL-60 cells with western Blot. Results The expression of the PTEN protein to be increased and the expression of the P-Akt protein to be inhibited of the HL-60 cells by UA with time dependent manner. this effect to be blocked by GW9662 of PPARγ pre-incubated. Conclusion The proliferation to be inhibited and the apoptosis to be inducted by Ursolic acid with related by raising the PTEN and inhibiting the p-AKt protein.of the HL-60 cells.