右美托咪定对缺血再灌注损伤大鼠心肌VEGF表达的影响

Effects of dexmedetomidine on expression of VEGF in myocardium of rats with myocardial ischemia-reperfusion injury

目的观察右美托咪定预处理对心肌缺血再灌注损伤(MIRI)大鼠VEGF表达的影响,探讨其对缺血再灌注心肌的保护作用。方法将40只健康雄性SD大鼠,随机分为4组:假手术组(A),MIRI组(B),MIRI+右美托咪定10μg/kg组(C),MIRI+右美托咪定30μg/kg组(D)。采用结扎冠脉左前降支的方法制作心肌缺血再灌注模型,免疫组化法测定各组大鼠心肌组织中VEGF的表达。结果与A组相比,B组、C组VEGF表达均减少(P<0.05);与B组相比,C组、D组VEGF表达均增加(P<0.05);与C组相比,D组VEGF表达增加(P<0.05)。结论右美托咪定通过预处理增加心肌缺血再灌注损伤大鼠心肌VEGF的表达保护心肌,且其作用与剂量有关。

Objective To explore the effect of dexmedetomidine on the expression of vascular endothelial growth factor（ VEGF） in myocardium of myocardial ischemia-reperfusion injury rats. Methods Forty healthy male SD rats were randomly divided into 4 groups：sham group（ A）,MIRI group（ B）,MIRI ＋ Dex 10 μg / kg group（ C）,MIRI ＋ Dex 30 μg / kg group（ D）. The left anterior descending coronary artery was ligated for establishing the rat myocardial ischemia-reperfusion injury model in vivo. Immunohistochemistry was used to analyze the expression of VEGF. Results Compared with group A,the expression of VEGF was reduced in group B and group C（ P 〈0. 05）. Compared with group B,the expression of VEGF was increased in group C and group D（ P 〈0. 05）. The expression in group D was significantly higher than in group C（ P 〈0. 05）. Conclusion Preconditioning of dexmedetomidine can have protective effects on the myocardium by up-regulating the expression of VEGF.