脂肪来源干细胞膜片与支持材料结合能力的初步探讨

Adhesion and Proliferation of Adipose Derived Stem Cells Sheet on Support Material

目的:探讨大鼠脂肪来源干细胞体外构建细胞膜片方法,并分析其同牙本质磨片和CBB(ceramic bovine bone,CBB)颗粒的结合能力和增殖影响。方法:从出生7d大鼠体内提取脂肪组织并获得纯化的脂肪来源干细胞(ADSCs,adipose-derived mesenchymal stem cell),采用四唑盐(MTT)比色法检测ADSCs增殖能力的变化;ADSCs经多向诱导分化后检测其向成骨细胞、脂肪细胞、成软骨细胞分化能力;ADSCs体外构建细胞膜片后,电镜下观察ADSCs膜片与CBB颗粒和牙本质磨片的结合能力,MTT比色法检测ADSCs和ADSCs膜片在材料上的增殖能力。结果:纯化的ADSCs具有良好的增殖能力,经体外诱导培养后向成骨细胞、脂肪细胞、成软骨细胞分化,电镜结果表明ADSCs膜片与牙本质磨片结合能力优于CBB颗粒,ADSCs膜片在牙本质磨片上的增殖能力优于CBB颗粒。结论:体外培养的大鼠脂肪来源干细胞具有种子细胞的特性,体外构建细胞膜片后与牙本质磨片的结合能力和增殖能力高于CBB颗粒,为细胞膜片治疗牙周缺损奠定基础。

Objective：To construct cells sheet from rat adipose derived mesenchymal stem cells（ADSCs）,and investigate its adhesion and proliferation on dentin disc and ceramic bovine bone（CBB）.Methods：ADSCs were extracted from adipose tissue of mice at 7days of age.MTT was used to determine the proliferation capacity of ADSCs and ADSCs sheet on CBB and dentin disc.Multilineage differentiation of ADSCs into osteoblasts,adipocytes,and chondrocytes was also detected.The adhesion ability of ADSCs on CBB and dentin discs was examined by scanning electron microscope（SEM）.Results：Purified ADSCs cells had excellent proliferation capacity.They could differentiate into osteoblasts,adipocytes,and chondrocytes.SEM results showed that ADSCs sheet had better adhesion on dentin disc than on CBB paticles.Conclusion：ADSCs sheet has higher proliferation and adhesion ability on dentin disc than CBB particles,which lays the foundation for cells sheet to treat periodontal defects.