牙科用锆基非晶合金的细胞毒性及血液相容性研究

Cell Toxicity and Blood Compatibility of Zr Based Amorphous Alloy for Dentistry

目的:测定高强度、高弹性模量的新型口腔种植用锆基非晶合金的细胞毒性及血液相容性。方法:通过锆基非晶合金与新鲜人血接触进行血液相容性实验并采用MTT法研究锆基非晶合金的细胞毒性。结果:细胞毒性试验显示本研究制备的锆基非晶合金的细胞毒性反应分级为0级,无细胞毒性;血液相容性试验结果锆基非晶合金溶血率低,动态凝血时间较纯钛及Ti6Al4V延长,以PT、APTT为指标的抗凝血性能与纯钛、Ti6Al4V相当,血小板粘附试验中Zr-Cu-Al-Ag较少出现血小板黏附,团聚及伪足伸出现象。结论:Zr-Cu-Al-Ag拥有良好的细胞相容性、血液相容性,可以初步满足其作为口腔种植材料的基本应用要求。

Objective：To evaluate the cell toxicity and hemo-compatibility of the Zr based amorphous alloy with high strength and modulus of elasticity for dentistry.Methods：Zr based amorphous alloy was performed the hemolysis test by contacting with fresh human blood.MTT method was applied to study the cytotoxicity of this alloy.Results：The level of cell toxicity of Zr based amorphous alloy was 0,which meant no cell toxicity.The Zr based amorphous alloy had low hemolytic rate,long dynamic clotting time,similar anticoagulant capability to pure titanium and Ti6Al4 V.The platelet adhesion test implied that Zr-Cu-Al-Ag alloy had seldom pseudopodium phenomenon.Conclusion：Zr-Cu-Al-Ag alloy has adequate cytocompatibilty and blood compatibility,which might meet the basic need of oral implantation.