红鳍东方鲀稚鱼肠道可培养细菌的多样性

Culturable Bacterial Diversity in Intestine of Farmed Juvenile Puffer Fish Takifugu rubripes

使用2216E平板涂布法从肠道样品分离可培养细菌,通过16S r DNA测序鉴定细菌。所有分离的细菌分为变形菌门(包含γ-变形细菌纲和α-变形细菌纲)和厚壁菌门。其中以γ-变形细菌纲为优势(83.3%)。在属的水平,从肠道中共分离出弧菌属、希瓦氏菌属、假交替单胞菌属、亚硫酸杆菌属、芽孢杆菌属、Aliivibrio、发光杆菌属、科尔韦尔氏菌属8个属,其中弧菌属、希瓦氏菌属和假交替单胞菌属的种类占总数的70%。首次发现亚硫酸杆菌属和科尔韦尔氏菌属作为红鳍东方鲀稚鱼肠道菌群的一部分。

It was aimed to examine the bacterial community associated to intestinal mucus of farmed juvenile puffer fish Takifugu rubripes. Polymerase chain reaction and sequencing of partial 16 S r DNA were performed on DNA from bacteria cultivated on Zobell 2216 E. All the isolates were classified into two phyla,Proteobacteria and Firmicutes.Gamma-Proteobacterium comprised the dominant culturable intestinal microbiota（ 83. 3%）,and 8 genera of bacteria were isolated from the intestine,including Vibrio,Shewanella,Pseudoalteromonas,Sulfitobacter,Bacillus,Aliivibrio,Photobacterium,and Colwellia in which Vibrio,Shewanella,and Pseudoalteromonas accounted for 70%of the total. Sulfitobacter and Colwellia were the first reported as the intestinal bacterial microflora in puffer fish T.rubripes.