TiO_2纳米管负载聚六亚甲基胍促进种植体钛表面成骨分化性能的研究

The effect of polyhexamethylene guanidine hydrochloride loaded on TiO_2 nanotubes on promoting the osteogenic differentiation of the modified titanium surface

目的研究聚六亚甲基胍对体外培养大鼠骨髓基质干细胞增殖和成骨性分化的影响,探索Ti O2纳米管负载聚六亚甲基胍对种植体钛表面是否具有促成骨分化能力。方法将1×10-9、1×10-8、1×10-7、1×10-6、1×10-5kg/L浓度的聚六亚甲基胍盐酸盐溶液分别作用于大鼠骨髓基质干细胞,采用MTT法分析细胞增殖情况。在不加成骨诱导液培养条件下比较聚六亚甲基胍作用组和不加药对照组碱性磷酸酶染色强弱,采用实时荧光定量PCR分析成骨性分化标志基因表达差异。将聚六亚甲基胍负载到Ti O2纳米管修饰种植体钛表面后和单纯Ti O2纳米管修饰种植体钛表面组、酸洗微米级粗糙种植体钛表面组实时荧光定量PCR分析成骨性分化标志基因表达差异。结果聚六亚甲基胍无明显促进细胞增殖能力,1×10-6kg/L浓度下能够促进大鼠骨髓基质干细胞的成骨性分化,将聚六亚甲基胍以1×10-5kg/L的浓度负载到Ti O2纳米管后,显著提高了种植体钛表面的促成骨分化能力。结论 Ti O2纳米管负载聚六亚甲基胍能够促进种植体钛表面成骨性分化,具有潜在的促进骨结合作用。

Objective To evaluate the effect of polyhexamethylene guanidine on osteogenic differentiation of rat bone marrow stromal stem cells（ BMSCs）,and to explore the effect of polyhexamethylene guanidine loaded on Ti O2 nanotubes on promoting the osteogenic differentiation of the modified titanium surface. Methods Different concentrations of polyhexamethylene guanidine hydrochloride（ PHMG-Cl）（ 1 × 10- 9、1 × 10- 8、1 × 10- 7、1 × 10- 6、1 × 10- 5kg / L） were supplemented into the culture media of rat bone marrow stromal stem cells separately. The cell proliferation was assayed by MTT method. The osteogenic differentiation markers were analyzed by real-time PCR assay. Then certain concentration of polyhexamethylene guanidine hydrochloride was loaded on the Ti O2 nanotubes modified titanium surface,and the osteogenic differentiation markers were analyzed by real-time PCR. Results Polyhexamethylene guanidine didn＇t inhibit the proliferation of rat BMSCs until the concentration was up to 1 × 10- 5kg / L. Concentration of 1 × 10- 6kg / L enhanced the osteogenic differentiation of rat BMSCs. With polyhexamethylene guanidine hydrochloride being loaded on the Ti O2 nanotubes modified titanium surface,the ability of osteogenic differentiation was further enhanced. Conclusions Polyhexamethylene guanidine loaded on Ti O2 modified titanium surface promotes its osteogenic function in vitro,which indicates that it has the ability to enhance osseointegration in vivo.