非转移性黑色素瘤糖蛋白B对口腔鳞癌细胞增殖、侵袭影响的研究

Effect of GPNMB on proliferation and invasion of oral squamous cell carcinoma cells

目的研究非转移性黑色素瘤糖蛋白B(glycoprotein(transmembrane)nonmetastatic melanoma protein b,GPNMB)对口腔鳞癌细胞增殖、侵袭的影响。方法采用RT-PCR及Western blot法检测口腔鳞癌组织、口腔鳞癌细胞中GPNMB的表达变化;构建GPNMB-siRNA载体,转染细胞后分别采用MTT法、Transwell小室法观察其对Tca-8113细胞增殖、侵袭的影响。结果 GPNMB在口腔鳞癌组织及口腔鳞癌细胞中均呈现高表达;增殖实验结果表明GPNMB-siRNA可显著抑制Tca-8113增殖;细胞侵袭结果表明GPNMB-siRNA可显著抑制Tca-8113侵袭。结论 GPNMB在口腔鳞癌细胞的增殖和侵袭中起着重要作用。

Objective To investigate the effect of glycoprotein（ transmembrane） nonmetastatic melanoma protein B（ GPNMB） on proliferation and invasion of oral squamous cell carcinoma cells. Methods The expression levels of GPNMB in oral squamous cell carcinoma tissues and oral squamous cell carcinoma cells were determined by RT-PCR and Western blot,respectively. GPNMB-siRNA was constructed,and then transfected into Tca-8113 cells. MTT assay and transwell chamber were used to determine the effect of GPNMBsiRNA on Tca-8113 cell proliferation and invasion. Results GPNMB was up-regulated in oral squamous cell carcinoma tissues and oral squamous cell carcinoma cells. In addition,MTT assay indicated that the proliferation of Tca-8113 cells was significantly inhibited by GPNMB-siRNA,and GPNMB-siRNA also markedly inhibited Tca-8113 cell invasion. Conclusions GPNMB may play an important role in Tca-8113 cell proliferation and invasion,which laid the foundation for studying the mechanism of GPNMB in oral squamous cell carcinoma.