肿瘤睾丸抗原乳酸脱氢酶C蛋白表达对肾癌细胞功能影响的实验研究

Effect of cancer-testis antigen LDHC on the invasion and metastasis of renal cell carcinoma

目的研究肿瘤睾丸抗原LDHC蛋白表达对肾癌细胞侵袭迁移等生物学功能的影响。方法采用siRNA技术在肾癌细胞系Caki-1中降低乳酸脱氢酶C(LDHC)的表达,通过细胞侵袭和迁移实验研究LDHC蛋白表达水平对肾癌细胞侵袭迁移等生物学行为的影响。同时测定LDHC表达降低后,MMP-9蛋白的表达水平及培养基中乳酸的浓度。结果当LDHC表达水平下调后,肾癌细胞的侵袭迁移能力减弱,MMP-9蛋白表达水平及培养基中乳酸浓度减低,与空白组及阴性组比较差异显著(P<0.05)。结论 LDHC的表达对肾癌细胞的功能有一定的影响,LDHC的表达降低,肾癌细胞的侵袭迁移能力减弱。其可能机制为LDHC能促进MMP-9的表达和乳酸的产生,从而增强肾癌细胞侵袭及迁移能力。

Objective To investigate the effect of LDHC protein on the invasion and metastasis of renal cell carcinoma.Methods Small interfering RNA mediated gene silencing approach was used to knockdown LDHC in renal cell carcinoma.The malignant characters of transfected Caki-1 cells including activities of invasion and metastasis were analyzed using millicell chamber assay.Western blot was used to quantify protein levels of LDHC and MMP-9.Lactic acid concentration in culture medium was measured.Results siRNA effectively inhibited LDHC protein levels（P〈0.05）.With siRNA interfering cells,the invasion and metastasis abilities of cells were depressed（P〈0.05）.Lactic acid concentration was decreased.The protein level of MMP-9 was reduced.Conclusions The expression of LDHC is associated with the function of RCC cells,whose invasion and metastasis abilities are depressed.The possible mechanism is that LDHC may enhance the expression of MMP-9 and increase the concentration of lactic acid in culture medium.