粪肠球菌Ef-5摇瓶种子工艺的研究

Study of Seed Technology about Enterococcus faecalis Ef- 5

本研究鸡源粪肠球菌Ef-5的种子培养基及培养条件进行了优化。通过正交设计实验得到Ef-5的优化种子培养基：葡萄糖25g，蛋白胨25g，酵母粉5g，磷酸氢二钾2g，柠檬酸三钠4g，氯化镁1g，氯化钠2g，硫酸锰0．75g，碳酸钙1g，吐温-801mL，蒸馏水1000mL。最佳培养条件：接种菌龄14h，接种量2．5％，初始pH7．0，培养温度35％。优化后的种子工艺加快了菌株Ef-5的生长速度，并使摇瓶种子活性提升了38．5％。

This study of seed medium and culture conditions for Enterococcus faecalis Ef - 5 of chicken were optimized. By orthogonal experiment obtain the optimization of the seed medium ： glucose 25g, peptone 25g, yeast extract 5g, trisodium citrate 4g, K2HPO4 2g, MgCl2 1 g, NaCl 2g, MnSO4 0.75g, CaCO31g, tween - 80 1mL, H2O 1000mL. Optimum culture condition： inoculum age 14h,inoculum size 2.5%, pH 7.0, temperature 35 ℃. The seed technology of the optimized speed up the growth rate of Ef-5,and make the seed activity increased 38.5%.