摘要
目的探讨实时定量PCR技术对传染性单核细胞增多症婴儿的诊断价值。方法选取临床表现为发热的婴儿442例,其中48例传染性单核细胞增多症患儿为观察组,另选择50例健康儿童为对照组,应用实时定量PCR技术(RT-qPCR)检测外周血EB病毒DNA载体,对患儿进行外周血异型淋巴细胞及血清嗜异凝集反应,并对单个细胞EBV-DNA载量进行检测,全面对比分析EBV-DNA诊断的诊断效果。结果 1EBV-DNA阳性患儿中异型淋巴细胞>10%患儿39例,占69.6%,血清嗜异凝集反应阳性患儿37例,占66.1%;EBV-DNA阴性患儿中异型淋巴细胞>10%患者9例,占2.3%,血清嗜异凝集反应阳性患者7例,占1.8%,两组患者异型淋巴细胞>10%患者及血清嗜异凝集反应阳性患者占比例差异有统计学意义(P<0.01);2VCA-IgM阳性率最高,阳性率为87.5%(43/48),明显高于对照组(P<0.01)。结论 VCA-IgM与DNA联合检测能提高婴幼儿传染性单核细胞增多症诊断准确性。
Objective To investigate the value of virus serology and DNA combined detection in infants with infectious mononucleosis.Methods 442 cases baby with fever were selected,Among the 48 cases of infectious mononucleosis in children as the observation group,Another 50 healthy children as control group,Application of real time quantitative PCR（RT-qPCR）EB in peripheral blood virus DNA detection technology of external carrier,The children were given peripheral blood heterotypic lymphocyte and serum heterophile agglutination reaction,And on the single cell EBVDNA load testing,comprehensive comparative analysis of diagnostic effects of EBV-DNA diagnosis.Results The EBVDNA positive patients were atypical lymphocyte in 39 〉10% patients,accounted for 69.6%,Serum heterophile agglutination reaction was positive in 37 cases,accounted for 66.1%;EBV-DNA negative children with atypical lymphocyte in 9patients with10%,accounted for 2.3%,7patients with positive serum eosinophil ISO agglutination reaction,accounted for 1.8%,there were statistical significance between patients with atypical lymphocytes in patients with10%and serum eosinophil percentage patients with positive abnormal agglutination reaction in two groups（P〈0.01）;The highest positive rate of VCA-IgM,The positive rate was 87.5%（43/48）,Significantly higher than control group（P〈0.01）.Conclusion The combined detection of VCA-IgM and DNA can improve the diagnostic accuracy of infant infectious mononucleosis.
出处
《中国实验诊断学》
2015年第10期1696-1698,共3页
Chinese Journal of Laboratory Diagnosis
