低温胁迫下酸浆叶片蛋白差异分析

Analysis on Physalis Leaf Protein Differences Under Low Temperature Stress

为进一步研究酸浆抗寒机理,以25℃室温条件(对照)和5℃低温处理条件下酸浆幼苗叶片为材料,运用蛋白质双向电泳结合质谱技术分析低温胁迫下酸浆叶总蛋白差异表达。结果表明:表达量存在显著差异的蛋白质点有16个,与对照组相比,表达量增加的蛋白质点9个,表达量减少的蛋白质点6个,新增蛋白质点有1个。经过鉴定,这些差异表达的蛋白质分别属于代谢相关蛋白、信号转导蛋白、能量相关蛋白、结合蛋白、抗性相关蛋白等。

In order to provided more theoretical basis for studying the cold resistance mechanism of Physalis,taking Physalis seedling leaves as materials which had been processed under 25℃ room temperature（control）and 5℃low temperature,Physalis leaf protein difference expression was analyzed with two-dimensional electrophoresis of protein and mass spectrometry.The results showed that there were 16 protein spots which had significant variations in protein expression amount.Compared with the control group,9protein spots expression amount increased,6protein spots expression amount decreased and one new protein spot was found.Appraisal identified that these differentially expressed proteins respectively belonged to metabolism related protein,signal transduction protein,energy related protein,binding protein,resistance related proteins and so on.