MCP-1基因-2518A/G与GPx-1基因Pro198Leu多态性的交互作用与非酒精性脂肪性肝病的关系

Interaction of Polymorphisms of MCP-1 Gene-2518A/G and GPx-1 Gene Pro198 Leu in Nonalcoholic Fatty Liver Disease

【目的】探讨单核细胞趋化蛋白-1(MCP-1)基因-2518A/G与谷胱甘肽过氧化物酶-1(GPx-1)基因Pro198Leu多态性的交互作用及其与非酒精性脂肪性肝病(NAFLD)的关系。【方法】采用病例-对照研究的方法,以NAFL、NASH、NAFHC患者及健康对照者各600例的外周血白细胞为样本,利用聚合酶链反应(PCR)技术分析了MCP-1基因-2518A/G和GPx-1基因Pro198Leu多态性。【结果】-2518A/G(GG)基因型和Pro198Leu(LL)基因型频率分布分别为49.67%和49.83%(NAFL组)、63.50%和63.50%(NASH组)、75.17%和75.50%(NAFHC组)及23.50%和23.33%(对照组),上述基因型频率在各病例组与对照组之间有显著差异(P<0.01)。-2518A/G(GG)基因型患NAFLD的风险显著增加(ORNAFL=3.21;ORNASH=5.66;ORNAFHC=9.85)。Pro198Leu(LL)基因型者患NAFLD的风险也显著增加(ORNAFL=3.26;ORNASH=5.72;ORNAFHC=10.13)。基因突变的协同分析发现-2518A/G(GG)/Pro198Leu(LL)基因型者在NAFL、NASH、NAFHC组和对照组中的分布频率分别为39.17%、53.17%、65.33%和12.17%,经χ2检验有统计学差异(P<0.01)。-2518A/G(GG)/Pro198Leu(LL)基因型者患NAFLD的风险显著增加(ORNAFL=5.30;ORNASH=10.91;ORNAFHC=23.92)。-2518A/G(GG)和Pro198Leu(LL)基因型有交互作用(γ1 NAFL=3.50,γ2 NAFL=3.37;γ1 NASH=4.79,γ2 NASH=4.72;γ1 NAFHC=6.19,γ2 NAFHC=5.90)。【结论】-2518A/G(GG和)Pro198Leu(LL)基因型均是NAFLD的易患因素,基因多态性的交互作用增加了NAFLD的发病风险。

【Objective】 To investigate the interaction of polymorphisms of monocyte chemoattractant protein-1（MCP-1） gene-2518 A / G and glutathione peroxidase-1（GPx-1） gene Pro198 Leu in nonalcoholic fatty liver disease（NAFLD）. 【Methods】 The genetic polymorphisms of MCP-1 gene-2518 A / G and GPx-1 gene Pro198 Leu were analyzed by polymorphism-polymerase chain reaction（PCR） technique in peripheral blood leukocytes of 600 NAFL, 600 NASH and 600 NAFHC cases and 600 healthy persons.【Results】 The frequencies of-2518 A / G（GG） and Pro198Leu（LL） were 49.67%and 49.83% in NAFL cases, 63.50% and 63.50%in NASH cases, 75.17% and 75.50% in NAFHC cases and 23.50% and 23.33% in healthy controls respectively. Statistical tests showed significant difference in the frequencies between case group and control group（P〈0.01）. The risk of NAFLD with-2518 A / G（GG） was significantly higher than those of controls（ORNAFL= 3.21; ORNASH= 5.66; ORNAFHC= 9.85）. The individuals who carried with Pro198Leu（LL） had a high risk of NAFLD（ORNAFL= 3.26; ORNASH= 5.72; ORNAFHC=10.13）. Combined analysis of the polymorphisms showed that percentage of-2518 A / G（GG） / Pro198Leu（LL） in NAFL, NASH, NAFHC and control groups was39.17%, 53.17%, 65.33% and 12.17%, respectively（P〈0.01）. The people who carried with-2518 A / G（GG） / Pro198Leu（LL）had a high risk of NAFLD（ORNAFL= 5.30;ORNASH= 10.91; ORNAFHC= 23.92）, and statistical analysis suggested a positive interaction between-2518 A / G（GG）and Pro198Leu（LL） in increasing the risk of NAFLD（γ1 NAFL= 3.50, γ2 NAFL= 3.37; γ1 NASH= 4.79, γ2 NASH=4.72; γ1 NAFHC= 6.19, γ2 NAFHC= 5.90）. 【Conclusion】-2518 A / G（GG） and Pro198Leu（LL） cigarette smoking are the risk factors in NAFLD, and significant interactions between genetic polymorphisms of-2518 A / G and Pro198 Leu added the risk of NAFLD.